Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

NuCycl PI Kit

  • Product Code: 7033
  • Size: 50 Tests
  • Availability: In Stock In Stock
  • Price (USD): $242

Cat #

7033		 Quantity:      

Data Sheet

Product Name

NuCycl PI Kit

Synonyms

NuCycl PI Kit

Product Type

DNA Staining Kit

Applications

Flow Cytometry

Size

50 Tests

Price (USD)

$242

Background

NuCycl PI (Propidium Iodide) - DNA stain procedure. NuCycl PI allows the rapid isolation of nuclei from cells and tissues. NuCycl PI stains only DNA.

Customer Storage

Product should be stored at 4-8°C. DO NOT FREEZE

References

1. Thornthwaite, J.T., Thomas, R.A. High - Resolution DNA Measurements Using the Nuclear Isolation Medium, DAPI, with the RATCOM Flow Cytometer. Methods in Cell Biology 33:111-119, 1990.

2. Hornicek, F.J., Thornthwaite, J.T., Malinin, G.I., Seckinger, D., and Malinin, T.I. Evaluation of 4,6 - diamidino-2-phenylindole (DAPI) for Flow Cytometry of Blastogenesis.

3. Thornthwaite, J.T., et al. A Review of DNA Flow Cytometric Preparatory and Analytical Methods. Immunocytochemistry in Tumor Diagnosis. Ed. J. Russo. Martinus Nijhoff: Boston pp. 380-398, 1985.

4. Lee, G.M., Thornthwaite, J.T., and Rasch, E.M. Cytometric Comparisons of DNA Levels in Neuronal and Glial Cells of the Cerebellum. Cell Biochem. Function. 33:262-270, 1985.

5. Lee, G.M., Thornthwaite, J.T., and Rasch, E.M. Picogram Per Cell Determination of DNA by Flow Cytofluorometry. Anal. Biochem. 137:221-226, 1984.

6. Coulson, P.B., Thornthwaite, J.T., Wooley, T., Seckinger, D., and Sugarbaker, E.V. Prognostic Indicators Including DNA Histogram Type Receptor Content, and Staging Related to Human Breast Cancer Patient Survival. Cancer Research. 44:4187-4196, 1984.

7. Youngberg, G.A., Thornthwaite, J.T., and Fanzus, D. Cytologically Malignant Squamous-Cell Carcinoma of the Penis. J. Dermtol. Surg. Oncol. 9:474-479, 1983.

8. Evans, D., Thornthwaite, J.T., Ng A., and Sugarbaker, E.V. DNA Flow Cytometry of Human Pleural Effusions: Comparison with pathology for the Diagnosis of Malignancy. Anal. Quant. Cyto. 5:19-27, 1983.

9. Thornthwaite, J.T., Sugarbaker, E.V., Temple W.J. Preparations of Tissues for DNA Flow Cytometric Analysis. Cytometry. 1:229-237, 1980.

10. Allen, L.M., Thornthwaite, J.T. Studies on the Pharmacology and Cytokinetics of Imidazole Pyrazole (NSC-51143) with p815 Mastocytoma Cells. Cancer Research 40:4059-1063, 1980.

11. Thornthwaite, J.T., Allen, L.M. The Effect of Glutamine Analog, AT-125, on the Cell Cycle of MCF-7 and BT-20 Human Breast Carcinoma Cells Using DNA Flow Cytometry. Res. Comm. Chem. Path. 29:393-396, 1980.

12. Temple, W.J., Sugarbaker, E.V., Thornthwaite, J.T., Hensley, G.T., Ketcham, A.S. Correlation of Cell Cycle Analysis with Duke's Staging in Colon Cancer Patient. J. Surg. Res. 28:314-318, 1980.

13. Sugarbaker, E.V., Thornthwaite, J.T., Temple, W.J., Ketcham, A.S. Flow Cytometry: General Principals and Applications to Selected Studies in Tumor Biology. Int. Adv. Surg. Oncol. 2:125-153.

Product Specific References:

1. Nemeth, M.J., et al. 'Hmgb3 deficiency deregulates proliferation and differentiation of common lymphoid and myeloid progenitors.' Blood, 2005, 105, 627-634.

2. Bhanu, N.V., et al. 'A sustained and pancellular reversal of gamma-globin gene silencing in adult human erythroid precursor cells.' Blood, 2005, 105, 387-393.

3. Barrette, S., et al. 'Lentivirus-based vectors transduce mouse hematopoietic stem cells with similar efficiency to Moloney murine leukemia virus-based vectors.' Blood, 2000, 96, 3385-3391.


Product Specific References


This product has been used in:

1. Eriksson, M., et al, 'Recurrent de novo point mutations in lamin A cause Hutchinson-Gilford progeria syndrome' Nature 2003, 423, , 293-298