Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

News

Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

CD19 PE

  • Product Code: 0194
  • Size: 100 Tests
  • Availability: In Stock In Stock
  • Price (USD): $191

Cat #

0194		 Quantity:      

Data Sheet

Product Name

CD19 PE

Synonyms

CD19 PE

Host/Source

Mouse

Clone

1G9

Isotype

IgG1

Product Type

Single-Color Reagent

Reactivity

Human

Applications

Flow Cytometry

Purification

Protein A/G Chromatography

Size

100 Tests

Price (USD)

$191

Background

Identification of human B cells associated approximately with10% of peripheral blood lymphocytes, 95,000 M.W. surface antigen.

Formulation

Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Customer Storage

Product should be stored at 4-8°C. DO NOT FREEZE

Product Image

Image Legend

Peripheral blood lymphocytes stained with Exalpha?s CD19 PE Conjugated Antibody (Cat. No. 0194).

Database Links:

SwissProtP15391Human

References

1. Functional Properties of CD19+ B Lymphocytes Positively Selected from Buffy Coats by Immunomagnetic Separation. Funderud S., Erikstien B., Asheim H.C., Nustad K., Stokke T., Blomhoff H.K., Holte H, Smeland E.B., Eur. J. Immunol. 1990 Ja;20(1):201-6.

2. Thymic B Cells from Myasthenia Gravis Patients are Activated B Cells. Phenotypic and Functional Analysis. Leprince C., CohenKaminsky S., Berrih-Aknin S., Vernet-Der Garabedian B., Treton D., Galanaud P., Richard Y., J. Immunol. 1990 Oct., 145(7):2115-22.

3. Prognostic Significance of CD34 Expression in Childhood B Precursor Acute Lymphocytic Leukemia: A Pediatric Onocology Group Study. Borowitz MJ, Shuster JJ, Civin CI, Carrol AJ, Look AT, Behm FG, Land VJ, Pullen DJ, Crist WM, J. Clin. Onol. 1990 Au;8(8):1389-98.

4. Biphenotypic Acute Leukemia in Adults. Sulak LE, Clare CN, Morale BA, Hansen KL, Montiel MM, Am. J. Clin. Path. 1990 Ju;94(1):54-8.

5. Intersection of the Complement and Immune Systems: A Signal Transduction Complex of the B Lymphocyte Containing Complement Receptor type 2 and CD19. Matsumoto AK, Kopicky-Burd J., Carter Rh, Tuveson DA, Tedder TF, Fearon, DT, J. Exp. Med. 1991 Jan. 173(1):55-64.

6. Immunofluorescence Measurement in a Flow Cytometer using Low-Power Helium Neon Laser Excitation. Shapiro, H.M, Glazer, A.N., Christenson, L., Williams, J.M., and Strom, T. B. Cytometry 4,276, 1983.

7. Comparison of Helium Neon and Dye lasers for Excitation of Allophycocyanin. Loken, M.R., Kiej, J.F. and Kelly, K.,A. Cytometry 8, 96, 1987.


Product Specific References


This product has been used in:

1. Takebayashi, M., et al, 'Blood dendritic cells are decreased in acute graft-versus-host disease' Bone Marrow Transplantation 2004, 33, , 989-996