Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.


Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.


  • Product Code: 0162
  • Size: 100 Tests
  • Availability: In Stock In Stock
  • Price (USD): $165

Cat #

0162		 Quantity:      

Data Sheet

Product Name










Product Type

Single-Color Reagent




Flow Cytometry


Protein A/G Chromatography


100 Tests

Price (USD)



Identification of human NK cell antigen expressing the 50 -70 kDa M.W. surface antigen associated with the IgG Fc receptor III on NK cells and Neutrophils. CD16 is expressed on approximately 15% of peripheral blood lymphocytes and is present on all resting NK cells. CD16 may be expressed on CD3 T cells from certain individuals.


CD16=Derived from the hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells of BALB/c mice immunized with NK lymphocytes


Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Customer Storage

Product should be stored at 4-8°C. DO NOT FREEZE

Database Links:



1. CD16 on human gamma delta T lymphocytes: expression, function, and specificity for mouse IgG isotypes. Braakman E; van de Winkel JG; van Krimpen BA; Jansze M; Bolhuis RL Cell Immunol 1992 Aug;143(1):97-107

2. Fc gamma RIII activation is different in CD16+ cytotoxic T lymphocytes and natural killer cells. Uciechowski P; Gessner JE; Schindler R; Schmidt RE Eur J Immunol 1992 Jun;22(6):1635-8

3. Involvement of a metalloprotease in spontaneous and phorbol ester-induced release of natural killer cell-associated Fc gamma RIII (CD16-II). Harrison D; Phillips JH; Lanier LL J Immunol 1991 Nov 15;147(10):3459-65

4. CD16- CD56+ natural killer cells after bone marrow transplantation. Jacobs R; Stoll M; Stratmann G; Leo R; Link H; Schmidt RE Blood 1992 Jun 15;79(12):3239-44

5. Natural killer function in flow cytometry: identification of human lymphoid subsets able to bind to the NK sensitive target K562. Vitale M; Zamai L; Neri LM; Manzoli L; Facchini A; Papa S Cytometry 1991;12(8):717-22

6. In vitro responsiveness to interleukins and theophylline of CD16+, CD56- natural killer cells in a patient with chronic granular lymphocyte disorder. Bayle C; Vitte-Mony I; Lang P; Pico J; Hercend T; Bertoglio J Leukemia 1992 May;6(5):470-6

7. Signal transduction by Fc gamma RIII (CD16) is mediated through the gamma chain. Wirthmueller U; Kurosaki T; Murakami MS; Ravetch JV J Exp Med 1992 May 1;175(5):1381-90

Product Specific References

This product has been used in:

1. Tajima, Kenichirou et al, 'Immunomodulatory effects of cyclosporin A on human perioheral blood dendritic cell subsets' Immunology 2003, 108, 3, 321