Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

News

Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

CD8 PE

  • Product Code: 0084
  • Size: 100 Tests
  • Availability: In Stock In Stock
  • Price (USD): $191

Cat #

0084		 Quantity:      

Data Sheet

Product Name

CD8 PE

Synonyms

CD8*PE

Host/Source

Mouse

Clone

17D8

Isotype

IgG1

Product Type

Single-Color Reagent

Reactivity

Human

Applications

Flow Cytometry

Purification

Protein A/G Chromatography

Size

100 Tests

Price (USD)

$191

Background

Identification of human T cells suppressor/cytotoxic expressing the 32 kDa M.W. surface antigen.

Immunogen

CD8=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.

Formulation

Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Customer Storage

Product should be stored at 4-8°C. DO NOT FREEZE

Product Image

Image Legend

Peripheral blood lymphocytes stained with Exalpha?s CD8 PE Conjugated Antibody (Cat. No. 0084).

Database Links:

SwissProtP01732Human

References

1. Evolutionary Conservation of Surface Molecules that Distinguish T Lymphocyte Helper/Inducer and T Cytotoxic/Suppressor Subpopulations in Mouse and Man. Ledbetter,J.A., Evans,R.L., Lipinski,M., Cunningham-Rundles,C., Good,R.A. and Herzenberg,L.A., J. Exp. Med. 153,310, 1981.

2. Circulating Antigen-Specific Suppressor T Cells in a Healthy Woman: Mechanism of Action and Isolation with a Monoclonal Antibody. Engleman,E.G.,Benike,C.J.,and Evans,R.L., Clin. Res. 29, 365a 1981.

3. Induction of Immunoglobulin Secreting Cells in the Allogeneic Mixed Leukocyte Reaction: Regulation by Helper and Suppressor Lymphocyte Subsets in Man. Kotzin,B.L., Benike,C.J. and Engleman,E.G., J. Imm. 127,931,1981.

4. CD4 and CD8 molecules can physically associate with the same T-cell receptor. Gallagher,P.F., Fazekas de St. Groth,B., Miller, J.F.A.P., Proc. Nat. Acad. Sci. 1989,86:10044.

5. Immunofluorescence Measurement in a Flow Cytometer using Low-Power Helium Neon Laser Excitation. Shapiro, H.M, Glazer, A.N., Christenson, L., Williams, J.M., and Strom, T. B. Cytometry 4,276, 1983.

6. Comparison of Helium Neon and Dye lasers for Excitation of Allophycocyanin. Loken, M.R., Kiej, J.F. and Kelly, K.,A. Cytometry 8, 96, 1987.

Cy-5 + Portions of this product is manufactured under license from Carnegie Mellon University, U.S. Patent Number 5,268,486.

Product Specific References:

1. Gardner, J.P. et al. 'Robust, but transient expression of adeno-associated virus-transduced genes during human t lymphopoisis.' Blood, 1997, 90, 4854-4864.


Product Specific References


This product has been used in:

1. Sauermann, U., et al, 'Homozygosity for a Conserved Mhc Class II DQ-DRB Haplotype Is Associated with Rapid Disease Progression in Simian Immunodeficiency Virus?Infected Macaques: Results from a Prospective Study' Journal of Infectious Disease 2000, 182, , 716-724