Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

Mouse anti-M13 phage coat protein g8p

Antibodies recognising M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The monoclonal antibodies manufactured and supplied by Exalpha react with either the pIII (g3p) or pVIII (g8p) proteins of M13 filamentous bacteriophage. All antibodies are available in a purified format. The antibodies are fully validated and are suitable for a wide range of techniques including:

  • ELISA
  • Flow Cytometry
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
For more information, click here for our M13 Bacteriophage information page.

News

Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

Lysophosphatidic Acid Receptor 1 CT (EDG-2)

  • Product Code: c174p
  • Size: 100 µg
  • Price (USD): $438

Cat #

c174p		 Quantity:      

Product Name

Lysophosphatidic Acid Receptor 1 CT (EDG-2)

Synonyms

Endothelial Cell Differentiation Gene Receptor 2; Lysophosphatidic Acid Receptor 1

Host/Source

Rabbit

Isotype

IgG

Product Type

Polyclonal Antibody

Reactivity

Human

Applications

Western Blot

Purification

Ammonium Sulfate Precipitation

Size

100 µg

Price (USD)

$438

Background

Endothelial cell differentiation gene-2 (EDG-2) receptor is a high affinity receptor for lysophosphatidic acid (LPA). EDG-2, also known as lysophospholipid receptor A1, Vzg-1, mrec1.3 or GPCR26, like all other EDG receptors, couple multiple (3 or more) types of G proteins and transduce decreases in c[AMP]i through Gi and increases in [Ca2+]i by augmenting phospholipase C through Gq/11 and beta/gamma dimers and by induction of PI3 kinase, p125 FAK, phospholipase D by activating rho through G12/13. Human EDG-2 is present in high levels on oligodendrocytes and certain human malignant T cell lines. EDG-2 receptors (with EDG-4) may play a role in protecting cardiomyocytes from apoptosis induced by hypoxia and andrenergic stimulation.

Immunogen

Synthetic peptide derived from the C terminal of the EDG2 receptor.

Positive Control

RH7777 Cells Transfected with EDG2/LPA1protein

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

50

Database Links:

SwissProtQ92633Human

References

1. Fukushima, N., et al., 'A single receptor encoded by vzg-1/lpA1/edg-2 couples to G proteins and mediates multiple cellular responses to lysophosphatidic acid. Proc. Natl.Acad. Sci. USA 1998, 95, 6151-6156

2. Goetzl, E., et al., 'Lysophosphatidic acid and sphingosine 1-phosphate protection of T cells from apoptosis in association with suppression of Bax.' J. Immunol. 1999, 162, 2049-2056

3. Zheng, Y., et al. 'Lysophosphatidic acid receptor-selection effects on Jurkat T cell migration through a matrigel model basement membrane.' J. Immunol. 2001, 166, 2317-2322

4. Parrill, A.L., et al. 'Identification of Edg1 receptor residues that recognize sphingosine 1-phosphate.' J. Biol. Chem. 2000, 275, 39379-39384.


Product Specific References


This product has been used in:

1. Hu, Xiaojie, et al, 'Lysophosphatidic acid (LPA) protects primary chronic lymphocytic leukemia (CLL) cells from apoptosis through LPA receptor activation of the anti-apoptotic protein AKT/PKB' Journal of Biological Chemistry 2005, 280, , 9498-9508
2. Shida, Dai, et al, 'Aberrant expression of lysophosphatidic acid (LPA) receptors in human colorectal cancer' Laboratory Investigation 2004, 84, , 1352-1362
3. Shida, Dai et al, 'Lysophosphatidic Acid (LPA) Enhances the Metastatic Potential of Human Colon Carcinoma DLD1 Cells through LPA1' Cancer Research 2003, 63, , 1706-1711