Exalpha Biologicals, Inc.

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Exalpha Biologicals, Inc.

M13 bacteriophage (g3p)

  • Product Code: Z115M
  • Size: 1 mg
  • Price (USD): $304

Cat #

Z115M		 Quantity:      

Data Sheet

Product Name

M13 bacteriophage (g3p)

Synonyms

Coat protein A; g3p; Minor coat protein

Host/Source

Mouse

Clone

E1

Isotype

IgG2a

Product Type

Monoclonal Antibody

Reactivity

N/A

Applications

ELISA, Western Blot, Flow Cytometry, IHC

Purification

Protein A/G Chromatography

Size

1 mg

Price (USD)

$304

Background

The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.

Immunogen

Hybridoma produced by the fusion of splenocytes from mice immunized with isolated M13 phage coat proteins and mouse myeloma cells.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Data respresents absorbancy readings for A10B phage on rabbit IgG (A10B/IgG), A10B phage on BSA (A10B/BSA), streptavidin on rabbit IgG (SA/IgG) and streptavidin on BSA (SA/BSA) for each dilution of biotinylated anti-M13 monoclonal antibody.

Database Links:

SwissProtP69168M13 Phage

References

1- Van Wezenbeek P.M., Schoenmakers J.G.; Nucleotide sequence of the genes III, VI and I of bacteriophage M13; Nucleic Acids Res. 6:2799-2818(1979). 2- Van Wezenbeek P.M., Hulsebos T.J., Schoenmakers J.G.; Nucleotide sequence of the filamentous bacteriophage M13 genome: comparison with phage fd; Gene 11:129-148(1980). 3- Cleary J.M., Ray D.S.; Deletion analysis of the cloned replication origin region from bacteriophage M13; J. Virol. 40:197-203(1981). 4- Hines J.C., Ray D.S.; Construction and characterization of new coliphage M13 cloning vectors; Gene 11:207-218(1980). 5- Yanisch-Perron C., Vieira J., Messing J.; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors; Gene 33:103-119(1985). 6- Messing J.; New M13 Vectors for Cloning; Meth. Enzymol. 101:20 78(1983). 7- Sanger F., Nicklen S., Coulson A.R.; DNA sequencing with chain-terminating inhibitors; Proc. Natl. Acad. Sci. U.S.A. 74:5463-5468(1977). 8- Zoller M.J., Smith M.; Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors; Meth. Enzymol. 100:468-500(1983). 9- Hu N.T., Messing J.; The making of strand-specific M13 probes; Gene 17:271-277(1982). 10- Heidecker G., Messing J., Gronenborn B.; A versatile primer for DNA sequencing in the M13mp2 cloning system; Gene 10:69-73(1980). 11- Ebright R., Dong Q., Messing J.; Corrected nucleotide sequence of M13mp18 gene III; Gene 114:81-83(1992). 12- Hong G.F.; A method for sequencing single-stranded cloned DNA in both directions; Biosci. Rep. 1:243-252(1981). 13- Messing J.; Multipurpose cloning system based on the single-stranded DNA bacteriophage M13; Recombinant DNA Technical Bulletin NIH 2:43-48(1979).


Product Specific References


This product has been used in:

1. Sakai, Keiko et al, 'Isolation and Characterization of Phage-Displayed Single Chain Antibodies Recognizing Nonreducing Terminal Mannose Residues. 1. A New Strategy for Generation of Anti-Carbohydrate Antibodies' Biochemistry 2007, 46, 1, 253-262 2. Qi,Cai et al, 'Phage M13KO7 detection with biosensor based on imaging ellipsometry and AFM microscopic confirmation' Virus Research 2009, 140, , 79-84 3. Tragoolpua, Khajornsak et al, 'Generation of functional scFv intrabody to abate the expression of CD147 surface molecule of 293A cells' BMC Biotechnology 2008, 8, 5, Online only