Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

Calreticulin

  • Product Code: Z100P
  • Size: 250 µg
  • Availability: In Stock In Stock
  • Price (USD): $244

Cat #

Z100P		 Quantity:      

Data Sheet

Product Name

Calreticulin

Host/Source

Sheep

Isotype

IgG

Product Type

Polyclonal Antibody

Reactivity

Human, Mouse, Rat, Dog

Applications

Western Blot, Immunoprecipitation, Immunohistochemistry

Purification

Ammonium Sulfate Precipitation

Size

250 µg

Price (USD)

$244

Background

Calreticulin, a Ca2+ chaperone, is an abundant highly conserved calcium binding protein found in the lumen of the endoplasmic reticulum of eukaryotic cells. This lumen protein can be used to measure the intactness of membrane preparations. It is used as a marker for subcellular fractionation of cells.

Immunogen

Recombinant protein derived from the carboxy terminus of the calreticulin protein fused to KLH.

Positive Control

MDCK cells; MCF-7 cells. Suitable for use against recombinant proteins conjugated to OVA, GST, His tags and other.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

60

Product Image

Image Legend

Western blot using antibody at 1:5000 dilution on indicated samples. Immunofluorescence of MCF-7 cells stained with antibody at 1:50 dilution.

Database Links:

SwissProtP28490Canine
SwissProtP18418Rat
SwissProtP14211Mouse
SwissProtQ6IAT4Human

References

1. Kageyama, S., et al. 'Urinary calreticulin in the diagnosis of bladder urothelial carcinoma.' Int. J. Urol. 2009, 16, 481-486

2. Silerova, M., et al. 'Characterization, molecular cloning and localization of calreticulin in Eisenia fetida earthworms.' Gene 2007, 397, 169-177

3. Souto-Padron, T., et al. 'Immunocytochemical localisation of calreticulin in Trypanosoma cruzi.' Histochem. Cell Biol. 2004, 122, 563-569