Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.


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Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.


  • Product Code: Z100P
  • Size: 250 µg
  • Availability: In Stock In Stock
  • Price (USD): $244

Cat #

Z100P		 Quantity:      

Data Sheet

Product Name






Product Type

Polyclonal Antibody


Human, Mouse, Rat, Dog


Western Blot, Immunoprecipitation, Immunohistochemistry


Ammonium Sulfate Precipitation


250 µg

Price (USD)



Calreticulin, a Ca2+ chaperone, is an abundant highly conserved calcium binding protein found in the lumen of the endoplasmic reticulum of eukaryotic cells. This lumen protein can be used to measure the intactness of membrane preparations. It is used as a marker for subcellular fractionation of cells.


Recombinant protein derived from the carboxy terminus of the calreticulin protein fused to KLH.

Positive Control

MDCK cells; MCF-7 cells. Suitable for use against recombinant proteins conjugated to OVA, GST, His tags and other.


Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight


Product Image

Image Legend

Western blot using antibody at 1:5000 dilution on indicated samples. Immunofluorescence of MCF-7 cells stained with antibody at 1:50 dilution.

Database Links:



1. Kageyama, S., et al. 'Urinary calreticulin in the diagnosis of bladder urothelial carcinoma.' Int. J. Urol. 2009, 16, 481-486

2. Silerova, M., et al. 'Characterization, molecular cloning and localization of calreticulin in Eisenia fetida earthworms.' Gene 2007, 397, 169-177

3. Souto-Padron, T., et al. 'Immunocytochemical localisation of calreticulin in Trypanosoma cruzi.' Histochem. Cell Biol. 2004, 122, 563-569