Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

News

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Exalpha Biologicals, Inc.

Protein G, Recombinant

  • Product Code: X2813RP
  • Size: 1 mg
  • Availability: In Stock In Stock
  • Price (USD): $62

Cat #

X2813RP		 Quantity:      

Data Sheet

Product Name

Protein G, Recombinant

Host/Source

E.coli

Isotype

N/A

Product Type

Recombinant Protein

Reactivity

Human IgG, Rat IgG2a

Applications

Antibody Purification

Size

1 mg

Price (USD)

$62

Background

Recombinant streptococcal protein G lacking the albumin binding region thereby avoiding undesirable reactions with albumin, though the Fc binding domain is still present. The recombinant Protein G is produced in Escherichia coli using sequence from Streptococcus C1-C2-C3. The Protein G contains 196 amino acids (190-384) having a molecular mass of 21.6 kDa. The Protein-G migrates on SDS-PAGE around 32 kDa.

Immunogen

E.coli

Formulation

Provided as solution in phosphate buffered saline

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

21.6