Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

DICER1

  • Product Code: X2756P
  • Size: 100 µg
  • Price (USD): $249

Cat #

X2756P		 Quantity:      

Data Sheet

Product Name

DICER1

Synonyms

Endoribonuclease Dicer; DICER; HERNA

Host/Source

Rabbit

Isotype

IgG

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Human

Applications

Western blot, Immunohistochemistry, EIA

Purification

Antigen Immunoaffiinity Purification

Size

100 µg

Price (USD)

$249

Background

Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. Also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs.

Immunogen

Synthetic peptide derived from human DICER1 protein.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Immunohistochemical staining of FFPE human colon carcinoma tissue using DICER1 antibody (Cat. No. X2756P). Antibody used at 1 µg/ml and visualized using DAB. Pathologists Comments: Cytoplasmic staining on mucous cells.

Database Links:

SwissProtQ9UPY3Human

References

1. Zhang, H., et al. ‘Single processing center models for human Dicer and bacterial RNase III.’ Cell, 118, 57-68 (2004) 2. Meister, G., et al. ‘Identification of novel argonaute-associated proteins.’ Curr. Biol., 15, 2149-2155 (2005) 3. Haase, A.D., et al. ‘TRBP, a regu1ator of cellular PKR and HIV-1 virus expression, interacts with Dicer and functions in RNA silencing.’ EMBO Rep., 6, 961-967 (2005) 4. Kok, K.H., et al. ‘Human TRBP and PACT directly interact with each other and associate with dicer to facilitate the production of small interfering RNA.’ J. Biol. Chem., 282, 17649-17657 (2007) 5. Melo, S.A., et al. ‘A TARBP2 mutation in human cancer impairs microRNA processing and DICER1 function.’ Nat. Genet., 41, 365-370 (2009)