Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

Histone H2A.x

  • Product Code: X2753P
  • Size: 100 µg
  • Price (USD): $249

Cat #

X2753P		 Quantity:      

Data Sheet

Product Name

Histone H2A.x

Synonyms

H2AFX; H2AX

Host/Source

Rabbit

Isotype

IgG

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Human

Applications

Western blot, Immunohistochemistry, EIA

Purification

Antigen Immunoaffiinity Purification

Size

100 µg

Price (USD)

$249

Background

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Immunogen

Synthetic peptide derived from human histone H2a.x protein.

Positive Control

Human bladder, ovary, colon

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Immunohistochemical staining of FFPE human bladder tissue using Histone H2A.x antibody at 5 µg/ml and antigen retrieval at pH 6.2. Visualization using goat anti-rabbit HRP secondary antibody and DAB substrate. Pathologists Comments: Excellent nuclear staining on bladder. pH 9.5 staining shows better nuclear staining of the neoplastic cells.

Database Links:

SwissProtP16104Human

References

1. Paull, T.T., et al. ‘A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage’ Curr. Biol., 10, 886-895 (2000) 2. Kobayashi, J., et al. ‘NBS1 localizes to gamma-H2AX foci through interaction with the FHA/BRCT domain.’ Curr. Biol., 12, 1846-1851 (2002) 3. Stewart, G.S., et al. ‘MDC1 is a mediator of the mammalian DNA damage checkpoint.’ Nature, 421, 961-966 (2003) 4. Lukas, C., et al. ‘Mdc1 couples DNA double-strand break recognition by Nbs1 with its H2AX-dependent chromatin retention.’ EMBO J., 23, 2674-2683 (2004)