Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

DDX4

  • Product Code: X2750P
  • Size: 100 µg
  • Price (USD): $249

Cat #

X2750P		 Quantity:      

Data Sheet

Product Name

DDX4

Synonyms

Probable ATP-dependent RNA Helicase DDX4; MVH; DEAD (Asp-Glu-Ala-Asp) box polypeptide 4

Host/Source

Rabbit

Isotype

Ig

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Human

Applications

Western blot, Immunohistochemistry

Purification

Antigen Immunoaffiinity Purification

Size

100 µg

Price (USD)

$249

Background

DDX4 is an ATP-dependent DEAD-box RNA helicase found in the chromatoid body of the germ cells (1). This enzyme is specific to germ cells and is necessary for germ cell development (2). Mouse DDX4 was shown to interact with Dicer, suggesting a role in microRNA-mediated RNA silencing (1).

Immunogen

Synthetic peptide derived from the human DDX4 protein

Positive Control

Human testis tissue

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Immunohistochemical staining of FFPE human testis using DDX antibody at 5 µg/ml visualized using goat anti-rabbit-HRP secondary and DAB substrate at pH 6.2. Pathologist comment: Very good staining on germ cell testicular @ pH6.2, little background on tissue.

Database Links:

SwissProtQ9NQI0Human

References

1. Kotaja, N., et al. ‘The chromatoid body of male germ cells: similarity with processing bodies and presence of Dicer and microRNA pathway components.’ Proc. Natl. Acad. Sci., 103, 2647-2652 (2006) 2. Albamonte, M.S., et al. ‘The developing human ovary: immunohistochemical analysis of germ-cell-specific VASA protein, BCL-2/BAX expression balance and apoptosis.’ Hum. Reprod. 23, 1895-1901 (2008)