Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

Mouse anti-M13 phage coat protein g8p

Antibodies recognising M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The monoclonal antibodies manufactured and supplied by Exalpha react with either the pIII (g3p) or pVIII (g8p) proteins of M13 filamentous bacteriophage. All antibodies are available in a purified format. The antibodies are fully validated and are suitable for a wide range of techniques including:

  • ELISA
  • Flow Cytometry
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
For more information, click here for our M13 Bacteriophage information page.

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Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

5T4 Oncofetal Trophoblast glycoprotein

  • Product Code: X2738P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $355

Cat #

X2738P		 Quantity:      

Data Sheet

Product Name

5T4 Oncofetal Trophoblast glycoprotein

Synonyms

Trophoblast glycoprotein, TPBG, 5T4 oncofetal antigen, 5T4 oncotrophoblast glycoprotein, 5T4 oncofetal trophoblast glycoprotein, M6P1

Host/Source

Rabbit

Isotype

Ig

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA

Purification

Antigen Immunoaffinity Purification

Size

100 µg

Price (USD)

$355

Background

5T4 is a N-glycosylated transmembrane 72 kDa (420 AA) glycoprotein containing seven leucine-rich repeat regions. 5T4 is often referred to as an oncofetal antigen due to its expression in fetal trophoblast where it was first discovered or trophoblast glycoprotein (TPBG). 5T4 oncofetal antigen is defined by a monoclonal antibody originally raised against human placental trophoblast and which recognizes a 72 kD glycoprotein expressed in a variety of different carcinomas but detected only at very low levels in normal epithelia. Patterns of expression of 5T4 oncofetal antigen in colorectal carcinomas indicate a significant association with tumor metastasis. By IHC, 71% of the carcinomas demonstrate positive 5T4 immunoreactivity in adenocarcinoma cells and/or associated stromal tissue.5T4 is found in tumors including the colorectal, ovarian, and gastric. Its expression is used as a prognostic aid in these cases. 5T4 is widespread in malignant tumors throughout the various stages of their development. In assessing the 5T4 phenotype it has been determined that there is a significant correlation between 5T4 expression and advanced stage of disease (FIGO stages III and IV) (P < 0.001) and with poorly differentiated tumors (P = 0.036) compared to well or moderately differentiated tumors. Patients with tumors expressing 5T4 were are likely to respond well to adjuvant therapy and have a significantly worse outlook in terms of survival and disease-free interval.

Immunogen

Synthetic peptide derived from human 5T4 oncofetal antigen protein

Positive Control

Ovarian Cancer, gastric cancer, colorectal cancer.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Database Links:

SwissProtQ13641Human

References

1. Yoshihara, S., et al. '5T4 glycoprotein regulates the sensory input-dependent development of a specific subtype of newborn interneurons in the mouse olfactory bulb.' J. Neurosci. 2012, 32, 2217-2226.

2. Southgate, T.D., et al. 'CXCR4 mediated chemotaxis is regulated by 5T4 oncofetal glycoprotein in mouse embryonic cells.' PLoS One 2010, 5, e9982.

3. Shingler, W.H., et al. 'Identification and functional validation of MHC class I epitopes in the tumor-associated antigen 5T4.' Int. Immunol. 2008, 20, 1057-1066.