Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Exalpha Biologicals, Inc.

BCRP (ATP-binding cassette sub-family G member 2)

  • Product Code: X2731P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $355

Cat #

X2731P		 Quantity:      

Data Sheet

Product Name

BCRP (ATP-binding cassette sub-family G member 2)

Synonyms

Breast cancer resistance protein; CDw338; Mitoxantrane resistance-associated protein; Placenta-specific ATP-binding cassette transporter

Host/Source

Rabbit

Isotype

Ig

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Human

Applications

Immunohistochemistry (paraffin-embedded)

Purification

Antigen Immunoaffinity Purification

Size

100 µg

Price (USD)

$355

Background

BCRP acts as a xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.

Immunogen

Synthetic peptide derived from human BCRP protein

Positive Control

Highly expressed in placenta

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

72kDa

Product Image

Image Legend

Immunohistochemical staining of normal human testis tissue using BCRP antibody (Cat. No. X2731P) at 10 µg/ml and detected using anti-Rabbit HRP secondary antibody and visualized using DAB substrate and hematoxylin counterstain.

Database Links:

SwissProtQ9UNQ0Human

References

1. Zhou, W., et al. 'The ABC transporter Bcrp1/ABCG2 is expressed in a wide variety of stem cells and is a molecular determinant of the side-population phenotype.' Nat. Med. 2001, 7, 1028-1034.

2. Itoda, M., et al. ''Eight novel single nucleotide polymorphisms in ABCG2/BCRP in Japanese cancer patients administered irinotacan.' Drug Metab. Pharmacokinet. 2003, 18, 212-217.

3. Zamber, C.P., et al. 'Natural allelic variants of breast cancer resistance protein (BCRP) and their relationship to BCRP expression in human intestine.' Pharmacogenetics 2003, 13, 19-28.