Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

ADAM DEC1

  • Product Code: X2727P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $355

Cat #

X2727P		 Quantity:      

Data Sheet

Product Name

ADAM DEC1

Synonyms

A disintegrin and metalloproteinase domain-like protein decysin 1,ADAM-like protein decysin 1

Host/Source

Rabbit

Isotype

IgG

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Human

Applications

Western Blot, ELISA, Immunohistochemistry

Purification

Antigen Immunoaffiinity Purification

Size

100 µg

Price (USD)

$355

Background

ADAMDEC1 is expressed in the immune system, by dendritic cells and macrophages. It may play an important role in the control of the immune response and during pregnancy.1 Due to the partial lack of a disintegrin domain and the total lack of a cysteine-rich domain, this protein has been placed in a novel subclass of the ADAM gene family2.

Immunogen

Synthetic peptide derived from the human ADAMDEC1 protein.

Positive Control

Expressed highly in the small intestine and appendix, moderately in lymph node, mucosal lining of the colon, thymus, spleen and very weakly in the bone marrow. Predominantly expressed in dendritic cells (DC) of the germinal center. Weakly expressed in monocyte and highly expressed in macrophage. Absent in immature DC.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

52.7 kDa

Product Image

Image Legend

Staining of human tonsil sections with ADAM-DEC1 antibody (Cat. No. X2727P) at 2 µg/ml.

Database Links:

SwissProtO15204Human

References

1. UniProtKB/Swiss-Prot Acession # O15204

2. Bates, E.E. et. atl., The ADAMDEC1 (decysin) gene structure: evolution by duplication in a metalloprtease gene cluster on chromosome 8p12. Immunogenetics. 2002 May;54(2):96-105. Epub 2002 Mar 16,