Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.


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Exalpha Biologicals, Inc.

Corticotropin-releasing hormone receptor 2

  • Product Code: X2726P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $355

Cat #

X2726P		 Quantity:      

Data Sheet

Product Name

Corticotropin-releasing hormone receptor 2





Product Type

Antigen Immunoaffinity Purified Polyclonal




Western Blotting, ELISA, Immunohistochemistry


Antigen Immunoaffiinity Purification


100 µg

Price (USD)



CRF2 is a receptor for corticotropin releasing factor. Shows high-affinity CRF binding. Also binds to urocortin I, II and III. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Expression levels shown to be comparable in adipose tissues and the heart of humans. Expression in adipose tissues suggests a functional role for CRH in human fat.


Synthetic peptide derived from the N-terminal region of the CRF2R protein.

Positive Control

Human colorectal tissue


Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

48 kDa

Product Image

Image Legend

Staining of normal human colorectal tissue with CRFR1 antibody (Cat. No. X2726P) at 2 µg/ml.

Database Links:



1. Liaw, C.W., et al. 'Cloning and characterization of the human corticotropin-releasing factor-2 receptor complementary deoxyribonucleic acid.' Endocrinology 1996, 137, 72-77.

2. Isfort, R.J., et al. 'Discovery of corticotropin releasing factor 2 receptor selective sauvagine analogues for treatment of skeletal muscle atrophy.' J. Med. Chem. 2005, 48, 262-265.

3. Argiles, J.M., et al. 'Effects of CRF2R agonist on tumor growth and cachexia in mice implanted with Lewis lung carcinoma cells.' Muscle Nerve 2008, 37, 190-195.

4. Tao, J., et al. 'Activation of corticotropin-releasing factor 2 receptor inhibits Purkinje neuron P-type calcium currents via G(o)alpha-dependent PKC epsilon pathway.' Cell Signal 2009, 21, 1436-1443.

5. Seres, J., et al. 'Corticotropin-releasing hormone system in human adipose tissue.' J. Clin. Endocrin. & Metab. 2004, 89, 965-970.