Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

Calcium Homeostasis Modulator Protein 1

  • Product Code: X2723P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $355

Cat #

X2723P		 Quantity:      

Data Sheet

Product Name

Calcium Homeostasis Modulator Protein 1

Synonyms

CALHM1; FAM26C

Host/Source

Rabbit

Isotype

IgG

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Human

Applications

Western Blotting, ELISA

Purification

Ammonium Sulfate Precipitation

Size

100 µg

Price (USD)

$355

Background

CALHM1 controls cytosolic Ca2+ permeability and cytosolic Ca2+ concentration. It controls amyloid precursor protein (APP) proteolysis and aggregated amyloid-beta (Abeta) peptides levels in a Ca2+ dependent manner. However, no association between CALHM1 and Alzheimer?s risk is unclear. Member of the FAM26 family of proteins. CALHM1 is predominantly expressed in the adult brain. It has also been detected in retinoic acid-differentiated SH-SY5Y cells. Also colocalizes with HSPA5 at the endoplasmic reticulum.

Immunogen

Synthetic peptide derived from human CALHM1 protein.

Positive Control

Human hepatocellular carcinoma

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Staining on paraffin embedded human hepatocellular carcinoma sections. Primary Ab (immunoaffinity purified) at 10 µg/ml. Antigen retrieval used: 10 mM Na Citrate pH 6.0, 10 minutes pressure cooker method, developed with anti rabbit HRP and DAP substrate. Counterstained with methyl blue.

Database Links:

SwissProtQ8IU99Human

References

1. Dreses-Werringloer, U., et la. A Polymorphism in CALHM1 Influences Ca2+ Homeostasis, Ab Levels, and Alzheimer?s Disease Risk. Cell 2008, 133, 1149-1161

2. Bertram, L., et al. 'No association between CALHM1 and Alzheimer?s disease risk.' Cell 2008, 135(6), 993-994.

3. Cui, P.J., et al. 'CALHM1 P86L polymorphism is a risk for Alzheimer?s Disease in the Chinese population.' J. Alzheimers Dis. 2009, Epub ahead of