Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Exalpha Biologicals, Inc.

a1C Calcium Channel

  • Product Code: X2713P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $355

Cat #

X2713P		 Quantity:      

Data Sheet

Product Name

a1C Calcium Channel

Synonyms

RBC-II; Cav1.2; Calcium channel ( L type) voltage gated 1c; Cacna1c

Host/Source

Rabbit

Isotype

IgG

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Human

Applications

Western Blot

Purification

Antigen Immunoaffiinity Purification

Size

100 µg

Price (USD)

$355

Background

Voltage-sensitive calcium channels (VSCCs) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1c gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-ctx-GVIA) and omega-agatoxin-IVA (omega-aga-IVA). Calcium channels containing the alpha-1c subunit play an important role in excitation-contraction coupling in the heart.

Immunogen

Synthetic peptide derived from the rat alpha1C calcium channel conjugated to KLH

Positive Control

Normal human kidney tissue

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

Immunohistochemical staining of normal human kidney tissue with alpha1C calcium channel antibody (Cat. No. X2713P) at 15 µg/ml

Database Links:

SwissProtQ13936Human

References

1. Snutch,T.P et.al 'Distinct calcium channels are generated by alternative splicing and are differentially expressed in the mammalian' CNS Neuron 7 (1), 45-57 (1991)

2. Hansen, P.B. et.al 'Differential expression of T- and L-type voltage-dependent calcium channels in renal resistance vessels.' Circ Res;89(7):630-8 (2001)

3. Chung,Y.H. et.al 'Differential alterations in the distribution of voltage-gated calcium channels in aged rat cerebellum.' Brain Res 8;903(1-2):247-52.(2001)