Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

Acid Ceramidase 1

  • Product Code: X2668P
  • Size: 100 µg
  • Price (USD): $355

Cat #

X2668P		 Quantity:      

Data Sheet

Product Name

Acid Ceramidase 1

Synonyms

N-acylsphingosine amidohydrolase (Acid ceramidase) 1; ASAH1

Host/Source

Rabbit

Isotype

IgG

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Human, Rat, Mouse

Applications

Western Blotting, ELISA, Immunohistochemistry

Purification

Antigen Immunoaffiinity Purification

Size

100 µg

Price (USD)

$355

Background

Acid ceramidase is a lipid hydrolyase responsible for the degradation of ceramide into sphingosine and free fatty acids within lysosomes. It can also synthesize ceramide from sphingosine and free fatty acids as well. The reverse activity is pH dependent (6.0 vs 4.5, respectively). This suggests that the enzyme may have diverse functions within cells dependent on its subcellular location and the local pH. Recent studies have shown that acid ceramidase activity is aberrantly expressed in several human cancers and that it might be a useful drug target. As an example, inhibitors of enzyme activity have been shown to slow the growth of cancer cells alone or in combination with other established, anti-oncogenic treatments. Aberrant activity has also been shown in Alzheimer's disease and overexpression may prevent insulin resistant (Type II) diabetes by free fatty acids.

Immunogen

Synthetic peptide derived from the C terminal region of the ASAH1 protein.

Positive Control

Human heart tissue

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

44 kDa

Product Image

Image Legend

Immunohistochemical staining of human heart tissue using Acid Ceramidase 1 antibody at 10 µg/ml.

Database Links:

SwissProtQ53H01Human
SwissProtQ9WV54Mouse
SwissProtQ9EQJ6Rat

References

1. Park, J.H., et al. 'Acid ceramidase and human disease.' Biochim Biophys Acta 2006; 1758:2133-2138. 2. Zeidan, Y.H., et al. 'Acid ceramidase but not acid sphingomyelinase is required for tumor necrosis factor-{alpha}-induced PGE2 production.' J. Biol. Chem. 2006; 281:24695-24703. 3. Shtraizent, N., et al. 'Autoproteolytic cleavage and activation of human acid ceramidase.' J. Biol. Chem. 2008; 283:11253-11259.