Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Exalpha Biologicals, Inc.

Cysteine string protein (CSP) Alpha, DNAJC5 alpha

  • Product Code: X2667P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $355

Cat #

X2667P		 Quantity:      

Data Sheet

Product Name

Cysteine string protein (CSP) Alpha, DNAJC5 alpha

Synonyms

CSP, DnaJ homolog subfamily C member 5

Host/Source

Rabbit

Isotype

IgG

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Human, Rat, Mouse

Applications

Western Blot, ELISA

Purification

Antigen Immunoaffiinity Purification

Size

100 µg

Price (USD)

$355

Background

May have an important role in presynaptic function. May be involved in calcium-dependent neurotransmitter release at nerve endings. The amino terminus of CSP contains the J domain, a consensus sequence found on a DnaJ like protein which is a member of the heatshock protein family. Since heat shock proteins serve as chaperones that participate in protein folding and protein complex formation, CSP is believed to perform similar functions in the assembly of synaptic vesicle proteins which are essential to membrane trafficking. Evidence for a role of CSP in presynaptic neurotransmitter release has come from genetic and physiological study in which the Drosophila CSP null mutant fails to elicit synaptic potential upon high frequency stimulation. CSP null mutants have a lethal phenotype and for the small percentage of mutant flies that survive, they become paralyzed and die at elevated temperature.

Immunogen

Synthetic peptide derived from the human CSP protein.

Positive Control

Human brain, human melanoma; Expressed in tissue of pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

22 kDa

Product Image

Image Legend

Immunohistochemical staining of human melanoma tissue using affinity purified CSP alpha antibody (Cat. No. X2667P) at 15 µg/ml.

Database Links:

SwissProtP60904Mouse
SwissProtP60905Rat
SwissProtQ9H3Z4Human

References

1. Coppola T., Gundersen C., Widespread expression of human cysteine string proteins.
FEBS Lett. 391:269-272(1996) [PubMed: 8764987] [Abstract]