Exalpha Biologicals, Inc.

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Exalpha Biologicals, Inc.

Influenza A, H1N1 New Caledonia 20/99, Hemagglutinin, Recombinant (Full Length)

  • Product Code: X2565
  • Size: 10 µg
  • Price (USD): $388

Cat #

X2565		 Quantity:      

Data Sheet

Product Name

Influenza A, H1N1 New Caledonia 20/99, Hemagglutinin, Recombinant (Full Length)

Host/Source

Sf9 cells

Product Type

Recombinant Protein

Applications

Elisa, WB

Purification

? 90% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: ? 0.1ng/ug (IEU/ug).

Size

10 µg

Price (USD)

$388

Background

Influenza virus is a single-stranded RNA virus, segmented, 70nm in diameter and enveloped. Strains are described by geographic origin, strain number, year of isolation and hemagglutination (H) and neuraminidase (N) antigens.

Immunogen

Recombinant full-length H1N1 A/New Caledonia/20/99 (MW 72kD) is glycosylated with N-linked sugars and produced using baculovirus vectors in insect cells. The insect cells were infected with A9440.1a, recombinant baculovirus expressing recombinant H1N1 A/New Caledonia/20/99. H1N1 New Caledonia shows 90% similarity to the A/PR/8/34 amino acid sequence.

Positive Control

Recombinant full-length H1N1 A/New Caledonia/20/99 (MW 7.5kD) is glycosylated with N-linked sugars and produced using baculovirus vectors in insect cells. The insect cells were infected with A9440.1a, recombinant baculovirus expressing recombinant H1N1 A/New Caledonia/20/99. H1N1 New Caledonia shows 90% similarity to the A/PR/8/34 amino acid sequence.

Formulation

Supplied as a sterile solution in 10mM sodium phosphate, pH 7.2, 150mM sodium chloride.

Customer Storage

May be stored at 4°C for short-term only. For long-term storage, add 0.1% HSA or BSA, store at -20°C. Aliquots are stable for at least 6 months at -20°C.

Target Molecular Weight

72 kDa

References

1. Wrammert, J., et al. “Broadly cross-reactive antibodies dominate the human B cell response against 2009 pandemic H1N1 influenza virus infection.” J. of Exp. Med. (2011), 208, 181-193.