Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

APM2 (CT)

  • Product Code: X2385P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $355

Cat #

X2385P		 Quantity:      

Data Sheet

Product Name

APM2 (CT)

Synonyms

Adipose most abundant gene transcript 2 protein, apM-2, APM2, C10orf116

Host/Source

Rabbit

Isotype

IgG

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Human

Applications

Western Blot

Purification

Antigen Immunoaffiinity Purification

Size

100 µg

Price (USD)

$355

Background

APM2 is an ~8kDa protein which until recently was found only in the EST data base but never as an expressed protein. APM2 has since been shown to be associated with cisplatin resistance in cell lines. APM2 is not found in cisplatin sensitive cell lines. These data implicate APM2 in the development of cisplatin resistance in tumor cells.

Immunogen

Synthetic peptide derived from human APM2 protein

Positive Control

Adipose tissue, cornea and liver.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

7855 Da

Product Image

Image Legend

Immunohistochemical staining of normal human liver tissue using APM2 antibody (Cat. No. X2385P) at 15 µg/ml.

Database Links:

SwissProtQ15847Human

References

1. Scott, B.J., et al. APM2 is a novel mediator of cisplatin resistance in a variety of cancer cell types regardless of p53 or MMR status. Int. J. Cancer 2009, 125, 1193-1204