Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

Mouse anti-M13 phage coat protein g8p

Antibodies recognising M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The monoclonal antibodies manufactured and supplied by Exalpha react with either the pIII (g3p) or pVIII (g8p) proteins of M13 filamentous bacteriophage. All antibodies are available in a purified format. The antibodies are fully validated and are suitable for a wide range of techniques including:

  • ELISA
  • Flow Cytometry
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
For more information, click here for our M13 Bacteriophage information page.

News

Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

SMO (Smoothened Homolog)

  • Product Code: X2376P
  • Size: 100 µg
  • Price (USD): $258

Cat #

X2376P		 Quantity:      

Data Sheet

Product Name

SMO (Smoothened Homolog)

Synonyms

Smoothened homolog [Precursor], SMO, Gx protein, SMO, SMOH

Host/Source

Rabbit

Isotype

IgG

Product Type

Polyclonal Antibody

Reactivity

Human

Applications

Western blot

Purification

Ammonium Sulfate Precipitation

Size

100 µg

Price (USD)

$258

Background

SMO is a G protein-coupled receptor (GPCR) that putatively associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. The binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). In basal cell carcinoma there are two somatic mutations SMO, D473-> H473 and R562 ->Q562.

Immunogen

Synthetic peptide derived from the human SMO protein

Positive Control

Brain

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

86 kDa

Product Image

Image Legend

Western blot using SMO antibody (Cat. No. X2376P) on human brain lysate (Cat. No. X1633C). Lysate loaded at 15µg/lane. Antibody used at 10 µg/ml (1) and 5 µg/ml (2) dilution. Secondary antibody, mouse anti-rabbit-HRP (Cat. No. X1207M), used at 1:150K dilution.

Database Links:

SwissProtQ99835Human

References

1. Stone, D.M., et al. The tumour-suppressor gene patched encodes a candidate receptor for Sonic hedgehog.; Nature 384:129-134(1996).

2. Xie, J., et al. Activating Smoothened mutations in sporadic basal-cell carcinoma.; Nature 391:90-92(1998).

3. Liu, Y., et al. 'Fused-Costal2 protein complex regulates Hedgehog-induced Smo phosphorylation and cell-surface accumulation.' Genes Dev. 2007 Aug 1;21(15):1949-63.

4. Moraea, R.C., et al. 'Constitutive activation of smoothened (SMO) in mammary glands of transgenic mice leads to increased proliferation, altered differentiation and ductal dysplasia.' Development. 2007 Mar;134(6):1231-42.

5. Ruiz-Gomez, A., et al. 'The cell biology of Smo signalling and its relationships with GPCRs.' Biochim Biophys Acta. 2007 Apr;1768(4):901-12.