Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.


Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

SMO (Smoothened Homolog)

  • Product Code: X2333P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $355

Cat #

X2333P		 Quantity:      

Data Sheet

Product Name

SMO (Smoothened Homolog)


Smoothened homolog [Precursor], SMO, Gx protein, SMO, SMOH





Product Type

Antigen Immunoaffinity Purified Polyclonal




Western blot


Antigen Immunoaffiinity Purification


100 µg

Price (USD)



SMO is a G protein-coupled receptor (GPCR) that putatively associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. The binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). In basal cell carcinoma there are two somatic mutations SMO, D473-> H473 and R562 ->Q562.


Synthetic peptide derived from the human SMO protein

Positive Control



Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

86 kDa

Product Image

Image Legend

Western blot using SMO antibody (Cat. No. X2376P) on human brain lysate (Cat. No. X1633C). Lysate loaded at 15µg/lane. Antibody used at 10 µg/ml (1) and 5 µg/ml (2) dilution. Secondary antibody, mouse anti-rabbit-HRP (Cat. No. X1207M), used at 1:150K dilution.

Database Links:



1. Stone, D.M., et al. The tumour-suppressor gene patched encodes a candidate receptor for Sonic hedgehog.; Nature 384:129-134(1996).

2. Xie, J., et al. Activating Smoothened mutations in sporadic basal-cell carcinoma.; Nature 391:90-92(1998).

3. Liu, Y., et al. 'Fused-Costal2 protein complex regulates Hedgehog-induced Smo phosphorylation and cell-surface accumulation.' Genes Dev. 2007 Aug 1;21(15):1949-63.

4. Moraea, R.C., et al. 'Constitutive activation of smoothened (SMO) in mammary glands of transgenic mice leads to increased proliferation, altered differentiation and ductal dysplasia.' Development. 2007 Mar;134(6):1231-42.

5. Ruiz-Gomez, A., et al. 'The cell biology of Smo signalling and its relationships with GPCRs.' Biochim Biophys Acta. 2007 Apr;1768(4):901-12.