Exalpha Biologicals, Inc.

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Exalpha Biologicals, Inc.

SMO (Smoothened Homolog)

  • Product Code: X2333P
  • Size: 100 µg
  • Price (USD): $355

Cat #

X2333P		 Quantity:      

Data Sheet

Product Name

SMO (Smoothened Homolog)

Synonyms

Smoothened homolog [Precursor], SMO, Gx protein, SMO, SMOH

Host/Source

Rabbit

Isotype

IgG

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Human

Applications

Western blot

Purification

Antigen Immunoaffiinity Purification

Size

100 µg

Price (USD)

$355

Background

SMO is a G protein-coupled receptor (GPCR) that putatively associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. The binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). In basal cell carcinoma there are two somatic mutations SMO, D473-> H473 and R562 ->Q562.

Immunogen

Synthetic peptide derived from the human SMO protein

Positive Control

Brain

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

86 kDa

Product Image

Image Legend

Western blot using SMO antibody (Cat. No. X2376P) on human brain lysate (Cat. No. X1633C). Lysate loaded at 15µg/lane. Antibody used at 10 µg/ml (1) and 5 µg/ml (2) dilution. Secondary antibody, mouse anti-rabbit-HRP (Cat. No. X1207M), used at 1:150K dilution.

Database Links:

SwissProtQ99835Human

References

1. Stone, D.M., et al. The tumour-suppressor gene patched encodes a candidate receptor for Sonic hedgehog.; Nature 384:129-134(1996). 2. Xie, J., et al. Activating Smoothened mutations in sporadic basal-cell carcinoma.; Nature 391:90-92(1998). 3. Liu, Y., et al. 'Fused-Costal2 protein complex regulates Hedgehog-induced Smo phosphorylation and cell-surface accumulation.' Genes Dev. 2007 Aug 1;21(15):1949-63. 4. Moraea, R.C., et al. 'Constitutive activation of smoothened (SMO) in mammary glands of transgenic mice leads to increased proliferation, altered differentiation and ductal dysplasia.' Development. 2007 Mar;134(6):1231-42. 5. Ruiz-Gomez, A., et al. 'The cell biology of Smo signalling and its relationships with GPCRs.' Biochim Biophys Acta. 2007 Apr;1768(4):901-12.