Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

Mouse anti-M13 phage coat protein g8p

Antibodies recognising M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The monoclonal antibodies manufactured and supplied by Exalpha react with either the pIII (g3p) or pVIII (g8p) proteins of M13 filamentous bacteriophage. All antibodies are available in a purified format. The antibodies are fully validated and are suitable for a wide range of techniques including:

  • ELISA
  • Flow Cytometry
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
For more information, click here for our M13 Bacteriophage information page.

News

Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

Ceramide kinase (CERK) (IN)

  • Product Code: X2160P
  • Size: 10 Miniblots
  • Availability: In Stock In Stock
  • Price (USD): $464

Cat #

X2160P		 Quantity:      

Data Sheet

Product Name

Ceramide kinase (CERK) (IN)

Synonyms

CERK, DA59H18.2, DA59H18.3, DKFZP434E0211, EC 2.7.1.138, FLJ21430, FLJ23239, HCERK, KIAA1646, LK4, MGC131878

Host/Source

Rabbit

Isotype

IgG

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Human

Applications

Immunohistochemistry

Purification

Immunoaffinity Purification

Size

10 Miniblots

Price (USD)

$464

Background

Sphingolipids, in addition to being structural components of membranes, regulate cell-cell and cell-substrate interactions, proliferation, and differentiation. Members of this diverse group of lipids have emerged as a novel class of signaling molecules that also regulate phagocytosis. The mechanisms by which sphingolipids exert these effects remain incompletely defined. More than a decade ago, it was found that ceramide can be phosphorylated to ceramide 1-phosphate (C1P). Ceramide kinase (CERK) and its phosphorylated product ceramide 1-phosphate (C1P) are central players in inflammation and cancer. The product of CERK activity, ceramide 1-phosphate (C1P), has been reported to have mitogenic effects. C1P is a direct activator of cytosolic phospholipase A2 and is involved in arachidonic acid release. CERK is a mediator of Ca2+-dependent degranulation in mast cells. In both arachidonic acid release and mast cell degranulation, the intracellular elevation of Ca2+ is a central event that acts as a regulatory mechanism of CERK activity. C1P is found in brain synaptic vesicles, and plays a role in regulating the secretion of neurotransmitters. CERK activity exists in HL-60 cells where the C1P is derived from ceramide released from sphingomyelin. The expressed kinase has specific ceramide phosphorylating activity. CERKs exist in a variety of cellular organisms, including plants, nematodes, insects, and vertebrates.

Immunogen

Synthetic peptide derived from the internal domain of human CERK

Positive Control

Human brain

Formulation

Phosphate buffered saline containing 0.1% sodium azide

Customer Storage

Product should be stored at -70°C. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

CERK staining of formallin fixed paraffin embedded human brain (cortex) tissue at a dilution of 20 µg/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

Database Links:

SwissProtQ8TCT0Human

References

1. Lamour NF, Chalfant CE. ‘Ceramide-1-phosphate: The missing link in eicosanoid biosynthesis and inflammation.’ Mol Interv. 2005 Dec;5(6):358-67.

2.Kim JW, Inagaki Y, Mitsutake S, Maezawa N, Katsumura S, Ryu YW, Park CS, Taniguchi M, Igarashi Y. ‘Suppression of mast cell degranulation by a novel ceramide kinase inhibitor, the F-12509A olefin isomer K1.’ Biochim Biophys Acta. 2005 Dec 30;1738(1-3):82-90. Epub 2005 Nov 14.

3. Van Overloop H, Gijsbers S, Van Veldhoven PP. ‘Further characterization of mammalian ceramide kinase: substrate delivery and (stereo)specificity, tissue distribution, and subcellular localization studies.’
J Lipid Res. 2006 Feb;47(2):268-83. Epub 2005 Nov 3.

4. Chalfant CE, Spiegel S. ‘Sphingosine 1-phosphate and ceramide 1-phosphate: expanding roles in cell signaling.’ J Cell Sci. 2005 Oct 15;118(Pt 20):4605-12. Review.

5. Mitsutake S, Igarashi Y. ‘Calmodulin is involved in the Ca2+-dependent activation of ceramide kinase as a calcium sensor.’ J Biol Chem. 2005 Dec 9;280(49):40436-41. Epub 2005 Oct 3.

6. Wijesinghe DS, Massiello A, Subramanian P, Szulc Z, Bielawska A, Chalfant CE. ‘Substrate specificity of human ceramide kinase.’
J Lipid Res. 2005 Dec;46(12):2706-16. Epub 2005 Sep 18.

7.Kim TJ, Mitsutake S, Kato M, Igarashi Y. ‘The leucine 10 residue in the pleckstrin homology domain of ceramide kinase is crucial for its catalytic activity.’ FEBS Lett. 2005 Aug 15;579(20):4383-8.


Product Specific References


This product has been used in:

1. Barth, B.M., et al. ‘Ceramide Kinase Regulates TNFα-Stimulated NADPH Oxidase Activity and Eicosanoid Biosynthesis in Neuroblastoma Cells’ Cell Signal. (2012), 24, 1126-1133