Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
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Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
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Exalpha Biologicals, Inc.

5-HT5A Receptor (HTR5A)

  • Product Code: X2142P
  • Size: 50 µg
  • Availability: In Stock In Stock
  • Price (USD): $464

Cat #

X2142P		 Quantity:      

Data Sheet

Product Name

5-HT5A Receptor (HTR5A)

Synonyms

5-HT-5A, 5-HT5A, HTR5A, MGC138226

Host/Source

Rabbit

Isotype

IgG

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Human

Applications

Immunohistochemistry

Purification

Immunoaffinity Purification

Size

50 µg

Price (USD)

$464

Background

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins.

Immunogen

3rd cytoplasmic domain of human

Positive Control

Human brain.

Formulation

Phosphate buffered saline containing 0.1% sodium azide

Customer Storage

Product should be stored at -70°C. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

HTR5A staining of formallin fixed paraffin embedded human brain (cerebellum) tissue at a dilution of 10 µg/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

Database Links:

SwissProtP47898Human

References

1. Doly, S., et al. '5-HT5A receptor localization in the rat spinal cord suggests a role in nociception and control of pelvic floor musculature.' J. Comp. Neurol. 2004, 30, 316-329

2. Wang, Z.Y., et al. '5-HT5a receptors in the carotid body chemoreception pathway of rat.' Neurosci. Lett. 2000, 278, 9-12

3. Garcia-Alcocer, G., et al. 'Serotonin receptor 5-HT5A in rat hippocampus decrease by leptin treatment.' Neurosci. Lett. 2010, 486, 171-173.

4. Yosifova, A., et al. Case-control association study of 65 candidate genes revealed a possible association of a SNP of HTR5A to be a factor susceptible to bipolar disease in Bulgarian population. J. Affect Disord 2009, 117, 87-97

5. Schanen, N.C., et al. Assignment of the 5-hydroxytryptamine (serotonin) receptor 5A gene (HTR5A) to human chromosome band 7q36.1. Cytogenet. Cell. Genet. 1996, 72, 187-188