Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

5-HT7 Receptor (HTR7)

  • Product Code: X2141P
  • Size: 50 µg
  • Availability: In Stock In Stock
  • Price (USD): $464

Cat #

X2141P		 Quantity:      

Data Sheet

Product Name

5-HT7 Receptor (HTR7)

Synonyms

5-HT-7, 5-HT7, 5HT7, HTR7

Host/Source

Rabbit

Isotype

IgG

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Human

Applications

Immunohistochemistry

Purification

Immunoaffinity Purification

Size

50 µg

Price (USD)

$464

Background

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase.

Immunogen

N-terminal domain of human

Positive Control

Human brain

Formulation

Phosphate buffered saline containing 0.1% sodium azide

Customer Storage

Product should be stored at -70°C. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

HTR7 staining of formallin fixed paraffin embedded human brain (cortex) tissue at a dilution of 10 µg/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

Database Links:

SwissProtP34969Human

References

1. Grueb, M., et al. 'Serotonin (5-HT7) Receptor-Stimulated Activation of cAMP-PKA Pathway in Bovine Corneal Epithelial and Endothelial Cells.' Ophthalmic. Res. 2012, 48, 22-27.

2. Tonini, M., et al. '5-HT7 receptors modulate peristalsis and accommodation in the guinea pig ileum.' Gastroenterology 2005, 129, 1557-1566.

3. Russo, A., et al. '?In vitro? postnatal expression of 5-HT7 receptors in the rat hypothalamus: an immunohistochemical analysis.' Brain Res. Dev. Brain Res. 2005, 154, 211-216.

4. Meuser, T., et al. '5-HT7 receptors are involved in mediating 5-HT-induced activation of rat primary afferent neurons.' Life Sci. 2002, 71, 2279-2289.