Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
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Exalpha Biologicals, Inc.

Bombesin Receptor Subtype 3 (BRS3)

  • Product Code: X2128P
  • Size: 50 µg
  • Availability: In Stock In Stock
  • Price (USD): $464

Cat #

X2128P		 Quantity:      

Data Sheet

Product Name

Bombesin Receptor Subtype 3 (BRS3)

Synonyms

BRS-3, BRS3

Host/Source

Rabbit

Isotype

IgG

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Human

Applications

Immunohistochemistry

Purification

Immunoaffinity Purification

Size

50 µg

Price (USD)

$464

Background

BRS3 is classified as an Orphan-A Receptor and is highly similar to the bombesin receptors but has a very low affinity for bombesin itself. Mice lacking BRS3 develop metabolic defects and obesity, suggesting that BRS3 may be an important target for obesity research. In addition, BRS3 may be involved in diabetes and hypertension. Role in sperm cell division, maturation, or function. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Immunogen

1st cytoplasmic domain of human

Positive Control

Human uterine tissue.

Formulation

Phosphate buffered saline containing 0.1% sodium azide

Customer Storage

Product should be stored at -70°C. Aliquot to avoid freeze/thaw cycles

Product Image

Image Legend

BRS3 staining of formallin fixed paraffin embedded uterus (pregnant) tissue at a dilution of 10 µg/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

Database Links:

SwissProtP32247Human

References

1. Boyle, R.G., et al. 'The design of a new potent and selective ligand for the orphan bombesin receptor subtype 3 (BRS3).' J. Pept. Sci. 2005, 11, 136-141

2. Reitman, M., et al. 'Pharmacokinetics and Pharmacodynamics of MK-5046, a Bombesin Receptor Subtype-3 (BRS-3) Agonist, in Healthy Patients.' J Clin Pharmacol. 2011, epub

3. Schulz, S., et al. 'Immunohistochemical detection of bombesin receptor subtypes GRP-R and BRS-3 in human tumors using novel antipeptide antibodies.' Virchow Arch. 2006, 449, 421-427