Exalpha Biologicals, Inc.

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Exalpha Biologicals, Inc.

Neuron specific Enolase

  • Product Code: X2070M
  • Size: 200 µg
  • Price (USD): $249

Cat #

X2070M		 Quantity:      

Data Sheet

Product Name

Neuron specific Enolase

Synonyms

ENO2; Enolase 2, (gamma, neuronal); 2-Phospho-D-Glycerate Hydrolase

Host/Source

Mouse

Clone

NSE-P1

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

Human, Mouse, Chicken

Applications

ELISA, Western Blot, Immunohistochemistry

Purification

Protein A/G Chromatography

Size

200 µg

Price (USD)

$249

Background

Enolases have been characterized as highly conserved cytoplasmic glycolytic enzymes that may be involved in differentiation. Three isoenzymes have been identified, ? Enolase, ? Enolase and ? Enolase. ? Enolase expression has been detected on most tissues, whereas ? Enolase is expressed predominantly in muscle tissue and ? Enolase is detected only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway.

Immunogen

Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the human NSE protein and mouse myeloma Ag8563 cells. Sequence common in human, mouse and chicken.

Positive Control

Serum, brain, pancreas, liver, skeletal muscle and endocrine tumors. Expression stronger in tumor versus normal tissue.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

47 kDa

Product Image

Image Legend

Left and Center: Immunohistochemical staining of normal pancreas tissue (left) and small bowel tumor tissue (center) using NSE antibody (X2070M and X2071M). Right: Western blot analysis using NSE antibodies (X2070M and X2071M) on ? and ? enolase standards.

Database Links:

SwissProtP09104Human

References

1. Kang, J.L., et al. (1996). Immunoreactive neuron-specific enolase (NSE) is expressed in testicular carcinoma-in-situ. J. Pathol. 178(2):161-165. 2. Koch, M., et al. (2007). Plasma S100beta and NSE levels and progression in multiple sclerosis. J. Neurol. Sci. 252(2):154-158. 3. Oertel, M., et al. (2006). S-100B and NSE: markers of initial impact of subarachnoid haemorrhage and their relation to vasospasm and outcome. J. Clin. Neurosci. 13(8):834-840.