Exalpha Biologicals, Inc.

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Exalpha Biologicals, Inc.

Heterogeneous Nuclear Ribonucleoprotein K

  • Product Code: X2068M
  • Size: 200 µg
  • Price (USD): $249

Cat #

X2068M		 Quantity:      

Data Sheet

Product Name

Heterogeneous Nuclear Ribonucleoprotein K

Synonyms

hnRNP-K; CSBP; TUNP; dC-Stretch Binding Protein

Host/Source

Mouse

Clone

F45 P9 C7

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

Human, Mouse, Rat, Rabbit, Chicken

Applications

Western Blot, Immunohistochemistry

Purification

Protein A/G Chromatography

Size

200 µg

Price (USD)

$249

Background

Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription and pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA Polymerase II. There are approximately 20 known hnRNP proteins, which range in size from 34 kDa to 120 kDa, and their complexes are the major constituents of the spliceosome. The majority of hnRNP proteins components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm, such as hnRNP K. hnRNP K, a 65 kDa protein, recruits a variety of molecular partners through two K homologous (KH) domains, which are required for protein-protein interactions. hnRNP K also contains several potential phosphorylation sites, including Ser 302, the major site of PKCd phosphorylation, which are thought to regulate various cellular functions, including sequence-specific DNA binding, transcription, RNA binding and nucleocytoplasmic shuttling.

Immunogen

Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the C-terminal of the hnRNP-K protein and mouse myeloma Ag8563 cells. Sequence common in human, mouse, rat, rabbit and chicken

Positive Control

Colorectal cancer tissue, Calu6 and HT29 cell lysates

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

51 kDa

Product Image

Image Legend

Left and Center: Immunohistochemical staining of normal colon (left) and colon carninoma (center) using hnRNP-K antibody (Cat. No. X2068M). Right: Western blot using hnRNP-K antibody on Calu6 (1) and HT29 (2) cell lysates.

Database Links:

SwissProtP61978Human

References

1. Wei, C.C., et al. (2006). Heterogeneous nuclear ribonucleoprotein K modulates angiotensinogen gene expression in kidney cells. J. Biol. Chem. 281(35):25344-25355 2. Dzwonek, A., et al. (2006). The diverse involvement of heterogeneous nuclear ribonucleoprotein K in mitochondrial response to insulin. FEBS Lett. 580(7):1839-1845 3. Carpenter, B., et al. (2006). Heterogeneous nuclear ribonucleoprotein K is over expressed, aberrantly localised and is associated with poor prognosis in colorectal cancer. Br. J. Cancer. 95(7):921-927