Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Exalpha Biologicals, Inc.

Tissue Inhibitor of Matrix Metalloproteinase 1 (TIMP-1)

  • Product Code: X2058M
  • Size: 200 µg
  • Availability: In Stock In Stock
  • Price (USD): $232

Cat #

X2058M		 Quantity:      

Data Sheet

Product Name

Tissue Inhibitor of Matrix Metalloproteinase 1 (TIMP-1)

Synonyms

TIMP-1; CLG1; EPA; HCI

Host/Source

Mouse

Clone

2A5

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

Human, Macaque

Applications

ELISA, Immunohistochemistry, Western Blot

Purification

Protein A/G Chromatography

Size

200 µg

Price (USD)

$232

Background

TIMP-1, TIMP-2, TIMP-3 and TIMP-4 (for tissue inhibitor of metalloproteinases-1, -2, -3 and -4) complex with metalloproteinases such as collagenases, gelatinases and stromelysins, resulting in irreversible inactivation of the metalloproteinase. TIMP-1 was found to be identical to EPA (erythroidpotentiation activity). Parathyroid hormone has been shown to be a regulator of TIMP-2 in osteoblastic cells. TIMP-3 may be involved in regulating trophoblastic invasion of the uterus as well as in regulating remodeling of the extracellular matrix during the folding of epithelia, and in the formation, branching and expansion of epithelial tubes. TIMP-4 is most highly expressed in heart and low levels of TIMP-4 are expressed in liver, brain, lung, thymus and spleen.

Immunogen

Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the human TIMP-1 protein and mouse myeloma Ag8563 cells. Sequence common in monkey

Positive Control

Colon and gastric tissues. Stronger expression observed in tumor versus normal protein.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

22-30 kDa

Product Image

Image Legend

Left: Immunohistochemical staining of human colon carcinoma tissue using TIMP-1 antibody (Cat. No. X2058M). Right: Western blot using TIMP-1 antibody against recombinant human TIMP-1 proenzyme (400 ng/lane).

Database Links:

SwissProtQ95KL9Macaca
SwissProtP01033Human

References

1. Hoikkala, S., et al. (2006). Tissue MMP-2 and MMP-9 [corrected] are better prognostic factors than serum MMP-2/TIMP-2--complex or TIMP-1 [corrected] in stage [corrected] I-III lung carcinoma. Cancer Lett. 236(1):125-132

2. Bodden, M.K., et al. (1994). Functional domains of human TIMP-1 (tissue inhibitor of metalloproteinases). J. Biol. Chem. 269(29):18943-18952