Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

Mouse anti-M13 phage coat protein g8p

Antibodies recognising M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The monoclonal antibodies manufactured and supplied by Exalpha react with either the pIII (g3p) or pVIII (g8p) proteins of M13 filamentous bacteriophage. All antibodies are available in a purified format. The antibodies are fully validated and are suitable for a wide range of techniques including:

  • ELISA
  • Flow Cytometry
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
For more information, click here for our M13 Bacteriophage information page.

News

Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

Matrix Metalloproteinase 3 (MMP3)

  • Product Code: X2055M
  • Size: 200 µg
  • Availability: In Stock In Stock
  • Price (USD): $232

Cat #

X2055M		 Quantity:      

Data Sheet

Product Name

Matrix Metalloproteinase 3 (MMP3)

Synonyms

SL-1, Matrix metalloproteinase-3, MMP-3, Transin-1

Host/Source

Mouse

Clone

1B4

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

Human

Applications

ELISA, Western Blot, Immunohistochemistry

Purification

Protein A/G Chromatography

Size

200 µg

Price (USD)

$232

Background

The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including Collagen, gelatin, Fibronectin, Laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-3, MMP-10 and MMP-11 (also designated stromelysin-1, 2 and 3, respectively) activate procollagenase. MMP-3 activation of procollagenase can occur via two pathways. Direct activation by MMP-3 is slow and activation by MMP-3 in conjunction with tissue or plasma proteinases is rapid. MMP-10 is expressed in small intestine, and at lower levels in lung and heart. MMP-11 is specifically expressed in stromal cells of breast carcinomas and contributes to epithelial cell malignancies.

Immunogen

Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the C-terminus of the human MMP3 protein and mouse myeloma Ag8563 cells.

Positive Control

Oesophageal and gastric tissues. Expressed at higher levels in tumor.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

50 kDa (proenzyme)

Product Image

Image Legend

Left: Immunohistochemical staining of human gastric carcinoma tissue using MMP-3 antibody (Cat. No. X2055M). Right: Western blot of recombinant human MMP-3 proenzyme using MMP-3 antibody.

Database Links:

SwissProtP08254Human

References

1. Knauper, V., et al. (1996). Activation of human neutrophil procollagenase by stromelysin 2. Eur. J. Biochem. 235(1-2);187-191

2. Saus, J. et al. (1988). The complete primary structure of human matrix metalloproteinase-3. Identity with stromelysin. J. Biol. Chem. 263;6742-6745