Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

Cytochrome P450 2A6

  • Product Code: X2045M
  • Size: 200 µg
  • Price (USD): $249

Cat #

X2045M		 Quantity:      

Data Sheet

Product Name

Cytochrome P450 2A6

Synonyms

CYP2A6; CYP450 2A6 (EC 1.14.14.1), CYPIIA6, Coumarin 7-hydroxylase

Host/Source

Mouse

Clone

F16 P2 D8

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Purification

Protein A/G Chromatography

Size

200 µg

Price (USD)

$249

Background

The cytochrome P450 proteins (CYPs) are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. P450 enzymes are classified into subfamilies based on their sequence similarities. CYP2A6 is a liver enzyme that metabolizes a number of drugs and a variety of procarcinogens, though it is primarily responsible for the metabolism of nicotine, the major addictive agent in tobacco. CYP2A6 inactivates nicotine to cotinine, and then cotinine to 3-hydroxycotinine. Differences in CYP2A6 genotypes are related to nicotine dependence, and may influence smoking habits and withdrawal symptoms in individuals that are quitting smoking. This suggests that an individualized smoking cessation program may be designed based on CYP2A6 genotypes.

Immunogen

Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with Synthetic peptide conjugated to ovalbumin derived from the C-terminus of CYP2A6 and mouse myeloma Ag8563 cells. Antigen identical in CYP2A6, 2A7 and 2A13.

Positive Control

Normal human liver tissue.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

58 kDa

Product Image

Image Legend

Left: Western blot using CYP2A6 antibody (Cat. No. X2045M) on recombinant cytochrome p450 at 1 µg/ml. Right: Staining using CYP2A6 antibody on normal human liver tissue at 1 µg/ml.

Database Links:

SwissProtP05177Human

References

1. Kumarakulasingham M, et al. (2005). Cytochrome p450 profile of colorectal cancer: identification of markers of prognosis. Clin Cancer Res. May 15;11(10):3758-65 2. Swan, G.E. (2005). Nicotine metabolism: the impact of CYP2A6 on estimate of additive genetic influence. Pharmacogenet. Genomics 15(2);115-125 3. Higashi, E., et al. (2007). Inhibitory effects of neurotransmitters and steroids on human CYP2A6. Drug Metab Dispos. 35(4); 508-514 4. Audrain-McGovern, J., et al. (2007). The role of CYP2A6 in the emergence of nicotine dependence in adolescents. 119(1);264-274