Rat sphingosine kinase 1 catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (spp)(sphingosine + ATP = sphingosine 1-phosphate + ADP), a lipid mediator with both intra- and extracellular functions. also acts on d-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as d,l-threo-dihydrosphingosine, n,n-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. The long form of sphingosine kinase 1, an N-terminal extension, is responsible for FceRI-mediated calcium mobilization and may play a role in other ligand mediated signaling events.
Synthetic peptide derived from the rat sphingosine kinase 1, long form, protein.
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Western blot of rat kidney lysate (10 ug/lane), detection of endogenous Sphingosine kinase 1, long form using X2038P (3 ug/ml), HRP anti-rabbit was used at 1:75k, and developed with Pierce’s Super Signal West Femto.
1. Venkataraman, K., et al. 'Extracellular export of sphingosine kinase-1a contributes to the vascular S1P gradient.' Biochem. J., 2006, 397, 461-4712. Ohotski, J., et al. Expression of sphingosine 1-phosphate receptor 4 and sphingosine kinase 1 is associated with outcome in oestrogen receptor-negative breast cancer. Br. J. Can. (2012), 106, 1453-1459