Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

Sirtuin 1 (193-741)

  • Product Code: X2037E
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $361

Cat #

X2037E		 Quantity:      

Data Sheet

Product Name

Sirtuin 1 (193-741)

Synonyms

HDAC Type 3,-1; Silent mating type information regulation 2 homolog 1

Host/Source

E.coli

Product Type

Active Enzyme

Reactivity

Human

Applications

Enyzme activity assay standard

Size

100 µg

Price (USD)

$361

Background

Human Sirtuin 1 (GenBank Accession No. NM_012238), amino acids 193-741 withN-terminal GST tag, MW=87.2 kDa, expressed in E.coli expression system. Sirtuin 1 is a NAD-dependent deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates Lys-382 of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Involved in HES1- and HEY2-mediated transcriptional repression. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. May serve as a sensor of the cytosolic ratio of NAD(+)/NADH, which is essential in skeletal muscle cell differentiation. Despite some ability to deacetylate histones in vitro, such activity is either weak or inexistent in vivo. Inhibited by nicotinamide. Activated by resveratrol (3,5,4'-trihydroxy-trans-stilbene), butein (3,4,2',4'- tetrahydroxychalcone), piceatannol (3,5,3',4'-tetrahydroxy-trans-stilbene),Isoliquiritigenin(4,2',4'-trihydroxychalcone), fisetin (3,7,3',4'-tetrahydroxyflavone) and quercetin (3,5,7,3',4'- pentahydroxyfla.Ivone). 3

Formulation

Provided in 50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 20 mM glutathione, and 10% glycerol.

Customer Storage

Product should be stored at -80°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

87.2

Database Links:

GenBankNM_012238Human

References

1. Kolthur-Seetharam, U. et al (2006) Cell Cycle 5, 873-877.

2. Solomon, J.M. et al(2006) Mol. Cell. Biol. 26, 28-38.

3. UCSC Genome Browser-http://genome.ucsc.edu/., Specific for Sirtuin 1:
http://genome.ucsc.edu/cgi-bin/hg Gen db=hg18&hgg_gene=NM_012238&hgg_chrom=chr10&hgg_start=69314432&hgg_end=69348147, Accessed March 13, 2007.