Exalpha Biologicals, Inc.

Latest News

Exalpha Biologicals, Inc. is now a US distributor for Nordic-Mubio products

Nordic-MUbio develops and manufactures high quality antibody reagents for use in R&D. All our products are manufactured according to strict ISO 9001 quality guidelines, and our focus is on developing antibodies targetting Cell Adhesion, Nuclear and Cytoskeletal Proteins.

Nordic-MUbio is one of the leading antibody companies developing antibodies for zebrafish research – we currently have over 50 antibodies validated for immunofluorescence in zebrafish.

Learn More

Latest References

Akazawa, Y., et al. "Endoplasmic Reticulum Stress Contributes to Helicobacter Pylori VacA-Induced Apoptosis" PLoS ONE 8(12): e82322

Learn More

Homa, N.J., et al. "Epstein-Barr Virus Induces Global Changes in Cellular mRNA Isoform Usage That Are Important for the Maintenance of Latency" J. Virol., 2013, 87, 12291-12301

Learn More
Exalpha Biologicals, Inc.

Sirtuin 1 (193-741)

  • Product Code: X2037E
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $361

Cat #

X2037E		 Quantity:      

Data Sheet

Product Name

Sirtuin 1 (193-741)

Synonyms

HDAC Type 3,-1; Silent mating type information regulation 2 homolog 1

Host/Source

E.coli

Product Type

Active Enzyme

Reactivity

Human

Applications

Enyzme activity assay standard

Size

100 µg

Price (USD)

$361

Background

Human Sirtuin 1 (GenBank Accession No. NM_012238), amino acids 193-741 withN-terminal GST tag, MW=87.2 kDa, expressed in E.coli expression system. Sirtuin 1 is a NAD-dependent deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates Lys-382 of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Involved in HES1- and HEY2-mediated transcriptional repression. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. May serve as a sensor of the cytosolic ratio of NAD(+)/NADH, which is essential in skeletal muscle cell differentiation. Despite some ability to deacetylate histones in vitro, such activity is either weak or inexistent in vivo. Inhibited by nicotinamide. Activated by resveratrol (3,5,4'-trihydroxy-trans-stilbene), butein (3,4,2',4'- tetrahydroxychalcone), piceatannol (3,5,3',4'-tetrahydroxy-trans-stilbene),Isoliquiritigenin(4,2',4'-trihydroxychalcone), fisetin (3,7,3',4'-tetrahydroxyflavone) and quercetin (3,5,7,3',4'- pentahydroxyfla.Ivone). 3

Formulation

Provided in 50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 20 mM glutathione, and 10% glycerol.

Customer Storage

Product should be stored at -80°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

87.2

Database Links:

GenBankNM_012238Human

References

1. Kolthur-Seetharam, U. et al (2006) Cell Cycle 5, 873-877.

2. Solomon, J.M. et al(2006) Mol. Cell. Biol. 26, 28-38.

3. UCSC Genome Browser-http://genome.ucsc.edu/., Specific for Sirtuin 1:
http://genome.ucsc.edu/cgi-bin/hg Gen db=hg18&hgg_gene=NM_012238&hgg_chrom=chr10&hgg_start=69314432&hgg_end=69348147, Accessed March 13, 2007.