Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

Mouse anti-M13 phage coat protein g8p

Antibodies recognising M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The monoclonal antibodies manufactured and supplied by Exalpha react with either the pIII (g3p) or pVIII (g8p) proteins of M13 filamentous bacteriophage. All antibodies are available in a purified format. The antibodies are fully validated and are suitable for a wide range of techniques including:

  • ELISA
  • Flow Cytometry
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
For more information, click here for our M13 Bacteriophage information page.

News

Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

Sirtuin 1 (193-741)

  • Product Code: X2037E
  • Size: 100 µg
  • Price (USD): $361

Cat #

X2037E		 Quantity:      

Data Sheet

Product Name

Sirtuin 1 (193-741)

Synonyms

HDAC Type 3,-1; Silent mating type information regulation 2 homolog 1

Host/Source

E.coli

Product Type

Active Enzyme

Reactivity

Human

Applications

Enyzme activity assay standard

Size

100 µg

Price (USD)

$361

Background

Human Sirtuin 1 (GenBank Accession No. NM_012238), amino acids 193-741 withN-terminal GST tag, MW=87.2 kDa, expressed in E.coli expression system. Sirtuin 1 is a NAD-dependent deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates Lys-382 of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Involved in HES1- and HEY2-mediated transcriptional repression. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. May serve as a sensor of the cytosolic ratio of NAD(+)/NADH, which is essential in skeletal muscle cell differentiation. Despite some ability to deacetylate histones in vitro, such activity is either weak or inexistent in vivo. Inhibited by nicotinamide. Activated by resveratrol (3,5,4'-trihydroxy-trans-stilbene), butein (3,4,2',4'- tetrahydroxychalcone), piceatannol (3,5,3',4'-tetrahydroxy-trans-stilbene),Isoliquiritigenin(4,2',4'-trihydroxychalcone), fisetin (3,7,3',4'-tetrahydroxyflavone) and quercetin (3,5,7,3',4'- pentahydroxyfla.Ivone). 3

Formulation

Provided in 50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 20 mM glutathione, and 10% glycerol.

Customer Storage

Product should be stored at -80°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

87.2

Database Links:

GenBankNM_012238Human

References

1. Kolthur-Seetharam, U. et al (2006) Cell Cycle 5, 873-877.

2. Solomon, J.M. et al(2006) Mol. Cell. Biol. 26, 28-38.

3. UCSC Genome Browser-http://genome.ucsc.edu/., Specific for Sirtuin 1:
http://genome.ucsc.edu/cgi-bin/hg Gen db=hg18&hgg_gene=NM_012238&hgg_chrom=chr10&hgg_start=69314432&hgg_end=69348147, Accessed March 13, 2007.