Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

Mouse anti-M13 phage coat protein g8p

Antibodies recognising M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The monoclonal antibodies manufactured and supplied by Exalpha react with either the pIII (g3p) or pVIII (g8p) proteins of M13 filamentous bacteriophage. All antibodies are available in a purified format. The antibodies are fully validated and are suitable for a wide range of techniques including:

  • ELISA
  • Flow Cytometry
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
For more information, click here for our M13 Bacteriophage information page.

News

Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

Akt (pan)

  • Product Code: X1890P
  • Size: 200 µl
  • Price (USD): $396

Cat #

X1890P		 Quantity:      

Data Sheet

Product Name

Akt (pan)

Synonyms

Protein Kinase B, RAC-alpha

Host/Source

Rabbit

Isotype

IgG

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Human, Mouse .

Applications

Western Blot

Purification

Purified from rabbit serum by sequential epitope-specific chromatography.

Size

200 µl

Price (USD)

$396

Background

Akt, also known as Protein Kinase B (PKB) or RAC-α, is a 60 kDa serine/threonine kinase that plays an important role in multiple biological responses including metabolism, cell survival and growth by phosphorylating many proteins including GSK-3β, caspase 9, BAD, and the Forkhead Transcription Factor. Akt is activated by PI3K, which in turn can interact with proteins like FAK thereby linking activation to the cytoskeleton. Akt/PKB is constitutively active in many types of human cancer, and contributes to tumor progression by promoting angiogenesis and cell invasiveness. Akt is fully activated by phosphorylation of threonine 308 by PDK1 and serine 473 by PDK2.

Immunogen

A synthetic peptide derived from the caraboxy-terminus region of human Akt1

Positive Control

Postive Control: 3T3 L1 cell extracts

Formulation

Provided as solution in phosphate buffered saline pH 7.3 with 0.1% BSA, 0.05% sodium azide, and 50% glycerol

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

60 kDa

Product Image

Image Legend

Extracts of 3T3L1 cells were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with a 1:1000 (1) or 1:500 (2) dilution of the Akt/PKB pan antibody for two hours at room temperature in a 3% BSA-TBST buffer. After washing, the membrane was incubated with goat F(ab’)2 anti-rabbit IgG HRP-conjugate and signals were detected using the Pierce SuperSignal™ method.

Database Links:

SwissProtP31749Human

References

1. Beffert, U., et al. (2002) Reelin-mediated signaling locally regulates protein kinase B/Akt and glycogen synthase kinase 3beta. J. Biol. Chem. 277(51):49958-49964.
2. Hsu, J., et al. (2001) The AKT kinase is activated in multiple myeloma tumor cells. Blood 98(9):2853-2855.
3. Persad, S., et al. (2001) Regulation of protein kinase B/Akt-threonine-308 phosphorylation by integrin linked kinase (ILK): Critical roles for kinase activity and amino acids arginine-211 and threonine-343. J. Biol. Chem. 276(29):27462-27469.
4. Nelson, J.M. and D.W. Fry. (2001) Akt, MAPK (Erk1/2), and p38 act in concert to promote apoptosis in response to ErbB receptor family inhibition. J. Biol. Chem. 276(18):14842-14847.
5. Genot, E.M., et al. (2000) The T-Cell receptor regulates Akt (Protein Kinase B) via a pathway involving Rac1 and phosphotidylinositide 3-Kinase. Mol. Cell. Biol. 20(15):5469-5478.
6. Datta, S.R., et al. (1997) Akt phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery. Cell 91(2):231-241.
7. del Peso, L., et al. (1997) Interleukin-3-induced phosphorylation of BAD through the protein kinase Akt. Science 278(5338):687-689.
8. Dudek, H., et al. (1997) Regulation of neuronal survival by the serine-threonine protein kinase Akt. Science 275(5300):661-665.
9. Franke, T.F., et al. (1997) PI3K: downstream AKTion blocks apoptosis. Cell 88(4):435-437.
10. An, W., et al. (2005) Mechanism of zinc-induced phosphorylation of p70 S6 kinase and glycogen synthase kinase 3β in SH-SY5Y neuroblastoma cells. J. Neurochem. 92:1104-1115 (cites the use of cat. # 44-604G and 44-622G (discontinued).
11. Kumar, P., et al. (2004) p38 MAPK mediates γ-irradiation-induced endothelial cell apoptosis, and vascular endothelial growth factor protects endothelial cells through the phosphoinositide 3-kinase-Akt-Bcl-2 pathway. J. Biol. Chem. 279(41):43352-43360 (cites the use of cat. # 44-622G (discontinued), 44-680G and 44-684G).