Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.


Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.


  • Product Code: X1858P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $258

Cat #

X1858P		 Quantity:      

Data Sheet

Product Name



Extracellular Sulfatase-2





Product Type

Polyclonal Antibody




Western Blot


Ammonium Sulfate Precipitation


100 µg

Price (USD)



Sulf-2 exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Mammalian Sulfs are endoproteolytically processed and secreted into the extracellular space of transfected cells, where they exhibit both arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity against intact heparin. Though the molecular weight of full length human Sulf2 is 100455 Da (870 amino acids), the endogenous Sulf2 is found by western blot to be present at molecular weights of 24.4, 33.7 and 51-60 kDa. Higher molecular weight species may be found depending on tissue and/or cell type. Analysis of human tumor tissue and tumor cell lines suggests that HSulf-1 is misregulated in cancers. HSulf-1 is found in a variety of normal tissues but is down-regulated in tumor cell lines originating from ovarian, breast, pancreatic, renal, and hepatocellular carcinoma. Tumors formed by cells expressing Sulf demonstrate enhanced extracellular matrix deposition. The 6-O-sulfation of heparan sulfate of myeloma tumor cells may be a critical factor in determiningand regulating the in vivo growth of this cancer.


Synthetic peptide derived from the human Sulf2 protein.

Positive Control

Antibody tested on human brain lysate


Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

100455 Da

Product Image

Image Legend

Western blot using Exalpha?s antigen immunoaffinity purified anti-human Sulf2 antibody (0.15 ug/ml) on human brain lysate (20 ug/lane) and developed using goat anti rbbit HRP (1:30,000)Pierce?s Super Signal West Femto.

Database Links:



1. Lum, D.H., et al. 'Gene trap disruption of the mouse heparan sulfate 6-O-endosulfatase gene, Sulf2.' Mol. Cell. Biol. 2007, 27, 678-688