Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

phospho-Sphingosine Kinase 1,(pS225), Mouse Reactive

  • Product Code: X1849P
  • Size: 100 µg
  • Price (USD): $397

Cat #

X1849P		 Quantity:      

Data Sheet

Product Name

phospho- Sphingosine Kinase 1,(pS225), Mouse Reactive

Host/Source

Rabbit

Isotype

IgG

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Mouse

Applications

Western Blot

Purification

Antigen Immunoaffiinity Purification

Size

100 µg

Price (USD)

$397

Background

Activation of Sphingosine kinase (Sphk) increases the bioactive lipid, sphingosine 1-phosphate (S1P) and has been observed in a variety of cancer cells. Phosphorylation of sphingosine by sphingosine kinase (Sphk) is the rate-limiting step in the cellular synthesis of S1P. A principle activation event is the phosphorylation of serine 225 of sphiongosine kinase 1. Inhibition of Sphk1 phosphorylation and hence, Sphk activity is an important target for the development of drugs for cancer, inflammation, cardioprotection as well as a many other indications.

Immunogen

A synthetic peptide incorporating the serine 225 phosphorylation site of mouse sphingosie kinase 1

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

42.5

Product Image

Image Legend

Western blot using anti-mouse SphK1 (pS225) (Cat. No. X1849P) on mouse B-cell lysate. Antibody used at 1 µg/ml with phosphorylated blocking peptide (Cat. No. X1877B) (lane A) and without (laneB).

Database Links:

SwissProtQ8CI15Mouse

References

1. Kihara A, Ikeda M, Kariya Y, Lee EY, Lee YM, Igarashi Y, Sphingosine-1-phosphate lyase is involved in the differentiation of F9 embryonal carcinoma cells to primitive endoderm. J. Biol. Chem. 278 (2003) 14578-14585 2. Choi OH, Kim JH, Kinet JP, Calcium mobilization via sphingosine kinase in signalling by the FceRI antigen receptor. Nature 380 (1996) 634-636 3. Olivera A, Kohama T, Edsall L, Nava V, Cuvillier O, Poulton S, Spiegel S, Sphingosine kinase expression increases intracellular sphingosine-1-phosphate and promotes cell growth and survival. J. Cell Biol. 147 (1999) 545-558 4. Chun J, Goetzl EJ, Hla T, Igarashi Y, Lynch KR, Moolenaar W, Pyne S, Tigyi G, International union of pharacology. XXXIV. Lysophospholipid receptor nomenclature. Pharmacol. Rev. 54 (2002) 265-269 5. Hla T, Signaling and biological actions of sphingosine 1-phosphate. Pharmacol. Res. 47 (2003) 401-407 6. Gregorius K, Mouritsen S, Elsner HI, Hydrocoating: a new method for coupling biomolecules to solid phases. J. Immunol. Methods, 181 (1995) 65-73 7. Zhang JH, Chung TDY, Oldenburg KR, A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J. Biomol. Screening, 4 (1999) 67-73 8. Ohotski, J., et al. Expression of sphingosine 1-phosphate receptor 4 and sphingosine kinase 1 is associated with outcome in oestrogen receptor-negative breast cancer. Br. J. Can. (2012), 106, 1453-1459


Product Specific References


This product has been used in:

1. El-Shewy, H.M., et al. 'Low-Density Lipoprotein Induced Expression of Connective Tissue Growth Factor via Transactivation of Sphingosine 1-Phosphate Receptors in Mesangial Cells.' Molecular Endocrinology, May 2012, 26(5): 833-845.