Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.


Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.


  • Product Code: X1844K
  • Size: 1 Plate
  • Availability: In Stock In Stock
  • Price (USD): $361

Cat #

X1844K		 Quantity:      

Data Sheet

Product Name

Phospho- AKT ELISA

Product Type





1 Plate

Price (USD)



Akt is a general protein kinase capable of phosphorylating several known proteins including TBC1D4 (TBC1 domain family, member 4). Akt signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. One of the essential functions of Akt is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3 and (Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3 mediated phosphorylation and degradation of cyclin D1. Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles


1. Tibes, R., et al. 'PI3K/AKT pathway activation in acute myeloid leukaemias is not associated with AKT1 pleckstrin homology domain mutation.' Br. J. Haematol. 2008, 140, 344-347

2. Wang, C., et al. 'Abelson interactor protein-1 positively regulates breast cancer cell proliferation, migration, and invasion.' Mol. Cancer Res. 2007, 5, 1031-1039

3. Eckstein, N., et al. 'Epidermal growth factor receptor pathway analysis identifies amphiregulin as a key factor for cisplatin resistance of human breast cancer cells.' J. Biol. Chem. 2008, 283, 739-750

4. Amar, S., et al. 'Possible involvement of post-dopamine D2 receptor signalling components in the pathophysiology of schizophrenia.' Int. J. Neuropsychopharmacol. 2007, Aug. 6, 1-9

5. Deeb, D., et al. 'Curcumin [1,7-bis(4-hydroxy-3-methoxyphenyl)-1-6-heptadine-3,5-dione; C21H20O6] sensitizes human prostate cancer cells to tumor necrosis factor- related apoptosis-inducing ligand/Apo2L-induced apoptosis by suppressing nuclear factor-kappaB via inhibition of the prosurvival Akt signaling pathway.' J. Pharmacol. Exp. Ther. 2007, 321, 616-625.

Product Specific References

This product has been used in:

1. Yan, B., et al. ?Regulation of PTEN/Akt Pathway Enhances Cardiomyogenesis and Attenuates Adverse Left Ventricular Remodeling following Thymosin 4 Overexpressing Embryonic Stem Cell Transplantation in the Infarcted Heart.? PLoS One, 8, e75580 (2013)