- Product Code: X1844K
- Size: 1 Plate
- Price (USD): $361
Akt is a general protein kinase capable of phosphorylating several known proteins including TBC1D4 (TBC1 domain family, member 4). Akt signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. One of the essential functionsof Akt is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3? and ? (Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3? mediated phosphorylation and degradation of cyclin D1. Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
References1. Tibes, R., et al. 'PI3K/AKT pathway activation in acute myeloid leukaemias is not associated with AKT1 pleckstrin homology domain mutation.' Br. J. Haematol. 2008, 140, 344-3472. Wang, C., et al. 'Abelson interactor protein-1 positively regulates breast cancer cell proliferation, migration, and invasion.' Mol. Cancer Res. 2007, 5, 1031-10393. Eckstein, N., et al. 'Epidermal growth factor receptor pathway analysis identifies amphiregulin as a key factor for cisplatin resistance of human breast cancer cells.' J. Biol. Chem. 2008, 283, 739-7504. Amar, S., et al. 'Possible involvement of post-dopamine D2 receptor signalling components in the pathophysiology of schizophrenia.' Int. J. Neuropsychopharmacol. 2007, Aug. 6, 1-95. Deeb, D., et al. 'Curcumin [1,7-bis(4-hydroxy-3-methoxyphenyl)-1-6-heptadine-3,5-dione; C21H20O6] sensitizes human prostate cancer cells to tumor necrosis factor- related apoptosis-inducing ligand/Apo2L-induced apoptosis by suppressing nuclear factor-kappaB via inhibition of the prosurvival Akt signaling pathway.' J. Pharmacol. Exp. Ther. 2007, 321, 616-625.
Product Specific References
This product has been used in:1. Yan, B., et al. 'Regulation of PTEN/Akt Pathway Enhances Cardiomyogenesis and Attenuates Adverse Left Ventricular Remodeling following Thymosin ?4 Overexpressing Embryonic Stem Cell Transplantation in the Infarcted Heart.' PLoS One, 8, e75580 (2013)