Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

Mouse anti-M13 phage coat protein g8p

Antibodies recognising M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The monoclonal antibodies manufactured and supplied by Exalpha react with either the pIII (g3p) or pVIII (g8p) proteins of M13 filamentous bacteriophage. All antibodies are available in a purified format. The antibodies are fully validated and are suitable for a wide range of techniques including:

  • ELISA
  • Flow Cytometry
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
For more information, click here for our M13 Bacteriophage information page.

News

Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

Phospho-AKT ELISA

  • Product Code: X1844K
  • Size: 1 Plate
  • Availability: In Stock In Stock
  • Price (USD): $361

Cat #

X1844K		 Quantity:      

Data Sheet

Product Name

Phospho- AKT ELISA

Product Type

ELISA Kit

Applications

ELISA

Size

1 Plate

Price (USD)

$361

Background

Akt is a general protein kinase capable of phosphorylating several known proteins including TBC1D4 (TBC1 domain family, member 4). Akt signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. One of the essential functions of Akt is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β mediated phosphorylation and degradation of cyclin D1. Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

References

1. Tibes, R., et al. 'PI3K/AKT pathway activation in acute myeloid leukaemias is not associated with AKT1 pleckstrin homology domain mutation.' Br. J. Haematol. 2008, 140, 344-347

2. Wang, C., et al. 'Abelson interactor protein-1 positively regulates breast cancer cell proliferation, migration, and invasion.' Mol. Cancer Res. 2007, 5, 1031-1039

3. Eckstein, N., et al. 'Epidermal growth factor receptor pathway analysis identifies amphiregulin as a key factor for cisplatin resistance of human breast cancer cells.' J. Biol. Chem. 2008, 283, 739-750

4. Amar, S., et al. 'Possible involvement of post-dopamine D2 receptor signalling components in the pathophysiology of schizophrenia.' Int. J. Neuropsychopharmacol. 2007, Aug. 6, 1-9

5. Deeb, D., et al. 'Curcumin [1,7-bis(4-hydroxy-3-methoxyphenyl)-1-6-heptadine-3,5-dione; C21H20O6] sensitizes human prostate cancer cells to tumor necrosis factor- related apoptosis-inducing ligand/Apo2L-induced apoptosis by suppressing nuclear factor-kappaB via inhibition of the prosurvival Akt signaling pathway.' J. Pharmacol. Exp. Ther. 2007, 321, 616-625.


Product Specific References


This product has been used in:

1. Yan, B., et al. ‘Regulation of PTEN/Akt Pathway Enhances Cardiomyogenesis and Attenuates Adverse Left Ventricular Remodeling following Thymosin β4 Overexpressing Embryonic Stem Cell Transplantation in the Infarcted Heart.’ PLoS One, 8, e75580 (2013)