Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Exalpha Biologicals, Inc.

Collapsin response mediator protein 1 (IN)

  • Product Code: X1790P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $307

Cat #

X1790P		 Quantity:      

Data Sheet

Product Name

Collapsin response mediator protein 1 (IN)

Synonyms

Collapsin response mediator protein 1 / DRP-1 / dihydropyropyrimidinase-related protein 1

Host/Source

Rabbit

Isotype

IgG

Product Type

Antigen Immunoaffinity Purified Polyclonal

Reactivity

Human, Mouse

Applications

Western Blot

Purification

Antigen Immunoaffiinity Purification

Size

100 µg

Price (USD)

$307

Background

Collapsin-response mediator proteins (CRMPs) are highly expressed in the developing brain where they play major roles in axonal outgrowth, neurite differentiation, and apoptosis (1). Their continued expression in areas of high synaptic remodeling such as the cerebellum, hippocampus, and the olfactory system suggests that these proteins may also be involved in adult brain plasticity (2). CRMP-1 was initially identified as a dihydro-pyrimidinase expressed exclusively in brain (3); later studies have shown that it is involved with neurotrophin (NT) 3-induced neurite formation and outgrowth (4). CRMP-1 localization switches from axonal to somatodendritic when neurons reach functional maturity, suggesting that it is involved in early neuronal differentiation as well as in later processes related to the survival or death of the newly generated neurons (5).

Immunogen

CRMP1 antibody was raised against a 16 amino acid peptide from near the center of human CRMP1

Positive Control

Tissue: Brain Found in the cytoplasm

Formulation

Provided as solution in phosphate buffered saline with 0.02% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

62 kD

Product Image

Image Legend

Western blot analysis of CRMP1 in Caco-2 cell lysate with CRMP1 antibody at (A) 1, (B) 2 and (C) 4 µg/ml.

Database Links:

SwissProtAAH07613Human

References

1) Charrier E, Reibel S, Rogemond V, et al. Collapsin response mediator proteins (CRMPs): involvement in nervous system development and adult neurodegenerative disorders. Mol. Neurobiol. 2003; 28:51-64.
2) Cameron HA and McKay RD. Adult neurogenesis produces a large pool of new granule cells in the dentate gyrus. J. Comp. Neurol. 2001; 435:406-417.
3.) Hamajima N, Matsuda K, Sakata S, et al. A novel gene family defined by human dihydropyrimidinase and three related proteins with differential tissue distribution. Gene. 1996; 180:157-63.
4) Quach TT, Duchemin A-M, Rogemond V, et al. Involvement of collapsin response mediator proteins in the neurite extension induced by neurotrophins in dorsal root ganglion neurons. Mol. Cell. Neurosci. 2004; 25:433-43.
5.) Veyrac A, Giannetti N, Charrier E, et al. Expression of collapsin response mediator proteins 1, 2 and 5 is differentially regulated in newly generated and mature neurons of the adult olfactory system. Eur. J. Neurosci. 2005; 21:2635-48.


Product Specific References


This product has been used in:

1. Nilsen, J., et al, Estradiol In Vivo Regulation of Brain Mitochondrial Proteome Journal of Neuroscience 2007, 27, , 14069-14077