Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

PTP1B full length

  • Product Code: X1765E
  • Size: 20 µg
  • Availability: In Stock In Stock
  • Price (USD): $361

Cat #

X1765E		 Quantity:      

Data Sheet

Product Name

PTP1B full length

Host/Source

E.coli

Product Type

Active Enzyme

Reactivity

Human

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Size

20 µg

Price (USD)

$361

Background

Protein tyrosine phosphatase 1B (PTP1B) is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This ubiquitously expressed enzyme is anchored in the endoplasmic reticulum by its C-terminal end and has its catalytic regions exposed to the cytosol. PTP1B will dephosphorylate a wide variety of phosphoproteins, such as receptors for the growth factors insulin and epidermal growth factor (EGF), c-Src and beta-catenin. Of particular interest is the observation that PTP1B knock-out mice are resistant to high-caloric intake-induced obesity and have exaggerated insulin responses, suggesting that PTP1B may play an important role in regulating growth factor responsiveness.

Immunogen

Full-Length PTP1B (a.a. 1-436) with N-terminal GST-HIs tag expressed in E.coli expression system

Positive Control

The specific activity of mouse PTP1B was determined using pNPP. Application: Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors

Formulation

Provided in 25 mM Tris-HCl, 75 mM NaCl, pH 8.0, 0.05% Tween, 2 mM EDTA, 1 mM DTT and 50% glycerol, 10 mM glutathione

Customer Storage

Product should be stored at -80°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

76 kDa

Database Links:

SwissProtM31724Human

References

1. Frangioni, J.V., et al. (1992). The nontransmembrane tyrosine phophatase PTP-1B localizes to the endoplasmic reticulum via its 35 amino acid C-terminal sequence. Cell 68(3);545-560

2. Elchebly, M., et al (1999). Increased insulin sensitivity and obesity resistance in mice lacking the protein tyrosine phosphatase-1B gene. Science 283(5407);1544-1548