Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

Mouse anti-M13 phage coat protein g8p

Antibodies recognising M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The monoclonal antibodies manufactured and supplied by Exalpha react with either the pIII (g3p) or pVIII (g8p) proteins of M13 filamentous bacteriophage. All antibodies are available in a purified format. The antibodies are fully validated and are suitable for a wide range of techniques including:

  • ELISA
  • Flow Cytometry
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
For more information, click here for our M13 Bacteriophage information page.

News

Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

c-Met

  • Product Code: X1761E
  • Size: 10 µg
  • Price (USD): $361

Cat #

X1761E		 Quantity:      

Data Sheet

Product Name

c- Met

Host/Source

Sf9 cells

Product Type

Active Enzyme

Reactivity

Human

Applications

Enzyme Kinetic studies, screening inhibitors, selectivity profiling

Size

10 µg

Price (USD)

$361

Background

When HGF/SF activates c-Met, the first proteins to be activated downstream are Grb2 (growth factor receptor bound protein 2) and Gab 1 (growth factor receptor bound protein 2 associated binder 1). Grb2 in turn may activate a number of kinase pathways, including the pathway from Ras to Raf to Mek and to MAPK(mitogen-activated protein kinase). Gab 1 activates PI3K (phosphoinositide 3 kinase), which activates STAT3 (signal transducer and activator of transcription). c-Met activation also induces activation of beta catenin, a key component of the wnt pathway, which translocates into the nucleus and participates in transcription regulation. The HGF/c-Met pathway plays an important role in the development of cancer. First through the activation of key oncogenic pathways (Ras, PI3K/STAT3, beta catenin), secondly through endothelial cell proliferation (neoangiogenesis), thirdly through increased protease production and hence cell dissociation leading to metastasis.

Immunogen

Baculovirus infected Sf9 cells

Formulation

Provided in 50mM Tris (pH 7.5) solution containing 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol

Customer Storage

Product should be stored at -80°C. Aliquot to avoid freeze/thaw cycles

References

1. Giordano, S. et al., Oncogene, 1989, 4: 1383-1388.

2. Lyer, A. et al., Cell Growth Differ. 1990, 1: 87-95.

3. Emaduddin M. et al., Proc. Natl. Acad. Sci. U.S.A. 105 (7):2358-2362 (2008).

4. Drebber U. et al., Oncol Rep. 19(6):1477-83 (2008).