Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

Flt1 N-Terminal His Tag

  • Product Code: X1758E
  • Size: 10 µg
  • Availability: In Stock In Stock
  • Price (USD): $361

Cat #

X1758E		 Quantity:      

Data Sheet

Product Name

Flt1 N-Terminal His Tag

Synonyms

EGFR-1, Vascular permeability factor receptor, Tyrosine-protein kinase receptor FLT, Tyrosine-protein kinase FRT, Fms-like tyrosine kinase 1

Host/Source

Sf9 cells

Product Type

Active Enzyme

Reactivity

Human

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Size

10 µg

Price (USD)

$361

Background

Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis. Description: Human recombinant Flt1 (FMS-like tyrosine kinase), also known as VEGFR1 (Vascular endothelial growth factor receptor 1), GenBank Accession No. AF063657, a.a. 783-end with N-terminal His tag, MW=67.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Immunogen

Baculovirus infected Sf9 cells

Formulation

25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.

Customer Storage

Product should be stored at -80°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

67.5

Database Links:

SwissProtAF063657Human

References

1. Rahimi, N., et al. J. Biol. Chem., 275: 16986-16992, 2000

2. Shibuya, M. Int. J. Biochem. Cell Biol., 33: 409-420, 2001