Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.


Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

Cytokeratin 17

  • Product Code: X1736M
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $335

Cat #

X1736M		 Quantity:      

Data Sheet

Product Name

Cytokeratin 17







Product Type

Monoclonal Antibody


Human, Rat


Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry


Protein A/G Chromatography


100 µg

Price (USD)



Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 ? 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.


Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with cytoskeletal preparation from rat colon and mouse X63 Ag 8.653 myeloma cells.

Positive Control

This antibody reacts with cytokeratin 17 in basal layers of pseudo-stratified and transitional epithelia.


Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Database Links:



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Int J Cancer 42, 147-53.
2. Troyanovsky, S. M., et al. (1989). Patterns of expression of keratin 17 in human epithelia: dependency on cell position, J Cell Sci 93, 419-26.
3. Smedts, F., et al. (1990). Keratin expression in cervical cancer, Am J Pathol 141, 497-511.
4. Wetzels, R. H., et al. (1991). Basal cell-specific and hyperproliferation-related keratins in human breast cancer. Am J Pathol 138, 751-763.
5. Wetzels, R. H., et al. (1992). Laminin and type VII collagen distribution in different types of human lung carcinoma: correlation with expression of keratins 14, 16, 17 and 18. Histopathology 20, 295-303.
6. Smedts, F., et al. (1992). Basal-cell keratins in cervical reserve cells and a comparison to
their expression in cervical intraepithelial neoplasia. Am J Pathol 140, 601-612.
7. Smedts, F., et al. (1994). Detection of keratin subtypes in routinely processed cervical tissue: implications for tumour classification and the study of cervix cancer aetiology. Virchows Arch 425, 145-155.
8. Litvinov, S. V., et al. (1996). Expression of Ep-CAM in cervical squamous epithelia correlates with an increased proliferation and the disappearance of markers for terminal differentiation. Am J Pathol 148, 865-875.
9. Moll, I., Moll, R. (1991). Comparative cytokeratin analysis of sweat gland ducts and eccrine poromas. Arch Dermatol Res. 283, 300- 09.
10. De Jong, E., van Vlijmen, I., van Erp, P., Ramaekers, F., Troyanowsky, S., Van de Kerkhof, P. (1991). Monoclonal anti-keratin 17: A useful marker for anti-psoriatic therapies. Arch Dermatol Res 283, 480-82.
11. Demirkesen, C., Hoede, N., Moll, R. (1995). Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins. J Cutan Pathol 22, 518-35.
12. Moll, R., et al. (1995). Differenzierungsmarker bei gyn