Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Exalpha Biologicals, Inc.

Cytokeratin 10

  • Product Code: X1733M
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $335

Cat #

X1733M		 Quantity:      

Data Sheet

Product Name

Cytokeratin 10

Host/Source

Mouse

Clone

RKSE60

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

Human, Mouse, Rat, Canine

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$335

Background

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 ? 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Immunogen

Hybridoma produced by the fusion of splenocytes from mice immunized with cytokeratins from the human epidermis and mouse SP2/0 myeloma cells.

Positive Control

This antibody reacts exclusively with cytokeratin 10 which is present in keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Database Links:

SwissProtQ6EIZ0Canine
SwissProtQ6IFW6Rat
SwissProtP02535Mouse
SwissProtP13645Human

References

1. Ramaekers, F. C., et al. (1983). Antibodies to intermediate filament proteins in the immunohistochemical identification of human tumours: an overview, Histochem J 15, 691-713.
2. Puts, J. J., et al. (1985). Expression of cytokeratins in early neoplastic epithelial lesions of
the uterine cervix, Int J Gynecol Pathol 4, 300-13.
3. Broers, J. L., et al. (1986). Intermediate filament proteins in classic and variant types of small cell lung carcinoma cell lines: a biochemical and immunochemical analysis using a
panel of monoclonal and polyclonal antibodies, J Cell Sci 83, 37-60.
4. Ramaekers, F., et al. (1987). Tissue distribution of keratin 7 as monitored by a monoclonal antibody, Exp Cell Res 170, 235-49.
5. Van Muijen, G. N., et al. (1987). Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in fetal, newborn, and adult epidermis, Exp Cell Res 171,
331-45.
6. Ramaekers, F., et al. (1990). Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas, Am J Pathol 136, 641-55.
7. Schaafsma, H. E., et al. (1990). Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression, Am J Pathol 136, 329-43.
8. Smedts, F., et al. (1990). Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia, Am J Pathol 136, 657-68.