Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Exalpha Biologicals, Inc.

Cytokeratin 8

  • Product Code: X1732M
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $335

Cat #

X1732M		 Quantity:      

Data Sheet

Product Name

Cytokeratin 8

Host/Source

Mouse

Clone

M20

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$335

Background

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 ? 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with keratin 18. Keratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas.

Immunogen

Hybridoma produced by the fusion of splenocytes from mice immunized with a keratin isolated from the human breast carcinoma cell line MCF-7 and mouse myeloma cells.

Positive Control

Antibody reacts with glandular epithelial cells (endocrine and exocrine) as well as mesothelial cells of the digestive, respiratory and urogenital tract and most adenocarcinomas derived from these cells.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Database Links:

SwissProtP11679Mouse
SwissProtP05787Human

References

1. Van Muijen, G. N., et al. (1987). Coexpression of intermediate filament polypeptides in human fetal and adult tissues, Lab Invest 57, 359-69.
2. Van Muijen, G. N., et al. (1987). Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in fetal, newborn, and adult epidermis, Exp Cell Res 171, 331-45.
3. Schaafsma, H. E., et al. (1989). Distribution of cytokeratin polypeptides in epithelia of the adult human urinary tract, Histochemistry 91, 151-9.
4. Smedts, F., et al. (1990). Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia, Am J Pathol 136, 657-68.
5. Ramaekers, F., et al. (1990). Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas, Am J Pathol 136, 641-55.
6. Schaafsma, H. E., et al. (1990). Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression, Am J Pathol 136, 329-43.
7. Ivanyi, D., et al. (1990). Keratin subtypes in carcinomas of the uterine cervix: implications for histogenesis and differential diagnosis, Cancer Res 50, 5143-52.
8. Wetzels, R. H., et al. (1991). Basal cell-specific and hyperproliferation-related keratins in human breast cancer, Am J Pathol 138, 751-63.
9. Ku, N. O., et al. (2001). Keratin 8 mutations in patients with cryptogenic liver disease, N Engl J Med 344, 1580-7.
10. Waseem, A., Karsten, U., Leigh, I.M., Purkis, P., Waseem, H. and Lane, B. (2004). Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability. Biochemistry 43, 1283-95.