Exalpha Biologicals, Inc.

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Exalpha Biologicals, Inc. is now a US distributor for Nordic-Mubio products

Nordic-MUbio develops and manufactures high quality antibody reagents for use in R&D. All our products are manufactured according to strict ISO 9001 quality guidelines, and our focus is on developing antibodies targetting Cell Adhesion, Nuclear and Cytoskeletal Proteins.

Nordic-MUbio is one of the leading antibody companies developing antibodies for zebrafish research – we currently have over 50 antibodies validated for immunofluorescence in zebrafish.

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Exalpha Biologicals, Inc.

Cytokeratin 7

  • Product Code: X1731M
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $335

Cat #

X1731M		 Quantity:      

Data Sheet

Product Name

Cytokeratin 7

Host/Source

Mouse

Clone

OVTL 12/30

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

Human only

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$335

Background

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 ? 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Immunogen

Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a cytoskeletal preparation of OTN-11 ovarian carcinoma cells and SP2/0-Ag14 mouse myeloma cells.

Positive Control

Cytokeratin 7 is present in a subgroup of glandular epithelia and their tumors, as well as transitional epithelium and transitional carcinoma.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Database Links:

SwissProtP08729Human

References

1. Ramaekers, F., et al. (1990). Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas, Am J Pathol 136, 641-55.

2. van Niekerk, C. C., et al. (1991). Immunohistochemical demonstration of keratin 7 in routinely fixed paraffin-embedded human tissues, J Pathol 165, 145-52.

3. van Niekerk, C. C., et al. (1991). Marker profile of different phases in the transition of normal human ovarian epithelium to ovarian carcinomas, Am J Pathol 138, 455- 63.

4. van de Molengraft, F. J., et al. (1993). OV-TL 12/30 (keratin 7 antibody) is a marker of
glandular differentiation in lung cancer, Histopathology 22, 35-8.

5. van Niekerk, C. C., et al. (1997). Differentiation margins of ovarian tumor pathology: first incidences of epithelial ovarian tumors monitored by marker antibodies, Cancer Detect Prev 21, 247-57.

6. Torenbeek, R., et al. (1998). Value of a panel of antibodies to identify the primary origin of adenocarcinomas presenting as bladder carcinoma, Histopathology 32, 20-7.