Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

Mouse anti-M13 phage coat protein g8p

Antibodies recognising M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The monoclonal antibodies manufactured and supplied by Exalpha react with either the pIII (g3p) or pVIII (g8p) proteins of M13 filamentous bacteriophage. All antibodies are available in a purified format. The antibodies are fully validated and are suitable for a wide range of techniques including:

  • ELISA
  • Flow Cytometry
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
For more information, click here for our M13 Bacteriophage information page.

News

Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

Cytokeratin 4

  • Product Code: X1729M
  • Size: 100 µg
  • Price (USD): $335

Cat #

X1729M		 Quantity:      

Data Sheet

Product Name

Cytokeratin 4

Host/Source

Mouse

Clone

6B10

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

Human, Canine, Feline

Applications

Immunohistochemistry, Immunocytochemistry, Western Blot, Flow Cytometry

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$335

Background

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Immunogen

Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a cytokeratin preparation extracted from human esophagus and SP2/0 mouse myeloma cells.

Positive Control

Antibody reacts exlcusively with cytokeratin 4 which is present in non-cornifying squamous epithelium, including cornea and transitional epithelium. Cells in certain ciliated pseudo-stratified epithelia and ductal epithelia of various exocrine glands also react with this antibody.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Database Links:

SwissProtP19013Human

References

1. van Muijen, G. N., et al. (1986). Cell type heterogeneity of cytokeratin expression in complex epithelia and carcinomas as demonstrated by monoclonal antibodies specific for cytokeratins nos. 4 and 13, Exp Cell Res 162, 97-113.

2. Weikel, W., et al. (1987). Characterization of subcolumnar reserve cells and other epithelia of human uterine cervix. Demonstration of diverse cytokeratin polypeptides in reserve cells, Virchows Arch B Cell Pathol Incl Mol Pathol 54, 98-110.

3. Ivanyi, D., et al. (1992). Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells, Am J Vet Res 53, 304-14.

4. Vos, J. H., et al. (1992). Immunohistochemistry with keratin monoclonal antibodies in canine tissues: urogenital tract, respiratory tract, (neuro-)endocrine tissues, choroid plexus and spinal cord, J Vet Med 39, 721-40.

5. Ivanyi, D., et al. (1993). Cytokeratins as markers of initial stages of squamous metaplasia in feline mammary carcinomas, Am J Vet Res 54, 1095-102.

6. Corver, W. E., et al. (2000). Four-color multiparameter DNA flow cytometric method to study phenotypic intratumor heterogeneity in cervical cancer, Cytometry 39, 96-107.