Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Exalpha Biologicals, Inc.

alpha T-Catenin

  • Product Code: X1726M
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $355

Cat #

X1726M		 Quantity:      

Data Sheet

Product Name

alpha T- Catenin

Synonyms

Cadherin-associated protein

Host/Source

Mouse

Clone

892_24D2S

Isotype

IgG2a

Product Type

Monoclonal Antibody

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Immunoprecipitation, Western Blot

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$355

Background

Catenins are proteins that connect cadherin/ -catenin cell adhesion complexes to the actin cytoskeleton. Although originally discovered in testis, T-catenin is expressed in other tissues, the highest levels being observed in heart. Immunohistochemical analysis showed human Tcatenin localization at intercalated discs of cardiomyocytes and in peritubular myoid cells of testis. It is proven that T-catenin can functionally restore cell-cell adhesion in colon cancer cells lacking -catenins. This indicates that this protein is involved in the formation of specific cell-cell contacts in specific types of muscle cells. The encoded protein has about the same predicted size (100 kDa) as other -catenins to which it shows an overall amino acid identity of 57%. In cells transfected with T-catenin cDNA, interaction with -catenin was demonstrated by coimmunoprecipitation. This antibody is specific for T-catenin and does not cross react with E-catenin or N-catenin by Western blot.

Immunogen

Hybridoma produced by the fusion of splenocytes from C57BI/6 mice immunized with a synthetic peptide derived from the human T-catenin protein and SP2/0-Ag14 mouse myeloma cells.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

~100 kDa

Database Links:

SwissProtQ9UI47Human

References

1. Janssens, B., et al. 'alphaTcatenin: a novel tissue-specific beta-cateninbinding protein mediating strong cell-cell adhesion', J. Cell. Sci. 2001, 114, 3177-88.