Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery
Exalpha Biologicals, Inc.

alpha T-Catenin

  • Product Code: X1726M
  • Size: 100 µg
  • Price (USD): $355

Cat #

X1726M		 Quantity:      

Data Sheet

Product Name

alpha T- Catenin

Synonyms

Cadherin-associated protein

Host/Source

Mouse

Clone

892_24D2S

Isotype

IgG2a

Product Type

Monoclonal Antibody

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Immunoprecipitation, Western Blot

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$355

Background

Catenins are proteins that connect cadherin/?-catenin cell adhesion complexes to the actin cytoskeleton. Although originally discovered in testis, ?T-catenin is expressed in other tissues, the highest levels being observed in heart. Immunohistochemical analysis showed human ?Tcatenin localization at intercalated discs of cardiomyocytes and in peritubular myoid cells of testis. It is proven that ?T-catenin can functionally restore cell-cell adhesion in colon cancer cells lacking ?-catenins. This indicates that this protein is involved in the formation of specific cell-cell contacts in specific types of muscle cells. The encoded protein has about the same predicted size (100 kDa) as other ?-catenins to which it shows an overall amino acid identity of 57%. In cells transfected with ?T-catenin cDNA, interaction with ?-catenin was demonstrated by coimmunoprecipitation. This antibody is specific for ?T-catenin and does not cross react with ?E-catenin or ?N-catenin by Western blot.

Immunogen

Hybridoma produced by the fusion of splenocytes from C57BI/6 mice immunized with a synthetic peptide derived from the human ?T-catenin protein and SP2/0-Ag14 mouse myeloma cells.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

~100 kDa

Database Links:

SwissProtQ9UI47Human

References

1. Janssens, B., et al. 'alphaTcatenin: a novel tissue-specific beta-cateninbinding protein mediating strong cell-cell adhesion', J. Cell. Sci. 2001, 114, 3177-88.