Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.


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Exalpha Biologicals, Inc.

Ceramide Glycosyltransferase

  • Product Code: X1700P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $258

Cat #

X1700P		 Quantity:      

Data Sheet

Product Name

Ceramide Glycosyltransferase


Glucosylceramide synthase, GCS, UDP glucose N-acylsphingosine D glucosyltransferase, GLCT-1, UDP glucose ceramide glucosyltransferase





Product Type

Polyclonal Antibody




Western Blot, ELISA


Ammonium Sulfate Precipitation


100 µg

Price (USD)



May serve as a flippase as well as a glucosyltransferase that transfers glucose to ceramide. It Catalzes; UDP-glucose + N-acylsphingosine = UDP + D-glucosyl-N-acylsphingosine. Ceramide Glycosyltransferase is the first step in the Glycosphingolipid synthesis; first glycosylation step. Glucosylceramide synthase (GlcT) and lactosylceramide synthase (GalT) are key enzymes for the synthesis of major glycosphingolipids of vertebrates.


Synthetic peptide derived from human ceramide glycosyltransferase protein.

Positive Control

Found in all tissues examined.


Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight


Product Image

Image Legend

Western blot analysis using Ceramide Glycosyltransferase antibody (X1700P) on 7 ug of rat kidney lysate. Antibody used at 1 ug/ml. Visualized using Pierce West Femto substrate system. Secondary used at 1:75k dilution. Exposure for 60 seconds.

Database Links:



1. Expression cloning of a cDNA for human ceramide glucosyltransferase that catalyzes the first glycosylation step of glycosphingolipid synthesis.; Ichikawa S., Sakiyama H., Suzuki G., Hidari K.I.-P., Hirabayashi Y.; Proc. Natl. Acad. Sci. U.S.A. 93:4638-4643(1996).

2. van Vlerken, L.E., et al. ?Modulation of Intracellular Ceramide Using Polymeric Nanoparticles to Overcome Multidrug Resistance in Cancer.? Cancer Res., 67, 4843-4850, (2007).

Product Specific References

This product has been used in:

1. D'Angelo, G., et a, 'Glycosphingolipid synthesis requires FAPP2 transfer of glucosylceramide.' Nature 2007, 449, , 62-67