Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

Mouse anti-M13 phage coat protein g8p

Antibodies recognising M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The monoclonal antibodies manufactured and supplied by Exalpha react with either the pIII (g3p) or pVIII (g8p) proteins of M13 filamentous bacteriophage. All antibodies are available in a purified format. The antibodies are fully validated and are suitable for a wide range of techniques including:

  • ELISA
  • Flow Cytometry
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
For more information, click here for our M13 Bacteriophage information page.

News

Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Exalpha Biologicals, Inc.

Ceramide Glycosyltransferase

  • Product Code: X1700P
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $258

Cat #

X1700P		 Quantity:      

Data Sheet

Product Name

Ceramide Glycosyltransferase

Synonyms

Glucosylceramide synthase, GCS, UDP glucose N-acylsphingosine D glucosyltransferase, GLCT-1, UDP glucose ceramide glucosyltransferase

Host/Source

Rabbit

Isotype

N/A

Product Type

Polyclonal Antibody

Reactivity

Human

Applications

Western Blot, ELISA

Purification

Ammonium Sulfate Precipitation

Size

100 µg

Price (USD)

$258

Background

May serve as a flippase as well as a glucosyltransferase that transfers glucose to ceramide. It Catalzes; UDP-glucose + N-acylsphingosine = UDP + D-glucosyl-N-acylsphingosine. Ceramide Glycosyltransferase is the first step in the Glycosphingolipid synthesis; first glycosylation step. Glucosylceramide synthase (GlcT) and lactosylceramide synthase (GalT) are key enzymes for the synthesis of major glycosphingolipids of vertebrates.

Immunogen

Synthetic peptide derived from human ceramide glycosyltransferase protein.

Positive Control

Found in all tissues examined.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

44856

Product Image

Image Legend

Western blot analysis using Ceramide Glycosyltransferase antibody (X1700P) on 7 ug of rat kidney lysate. Antibody used at 1 ug/ml. Visualized using Pierce West Femto substrate system. Secondary used at 1:75k dilution. Exposure for 60 seconds.

Database Links:

SwissProtQ16739Human

References

1. Expression cloning of a cDNA for human ceramide glucosyltransferase that catalyzes the first glycosylation step of glycosphingolipid synthesis.; Ichikawa S., Sakiyama H., Suzuki G., Hidari K.I.-P., Hirabayashi Y.; Proc. Natl. Acad. Sci. U.S.A. 93:4638-4643(1996).

2. van Vlerken, L.E., et al. ‘Modulation of Intracellular Ceramide Using Polymeric Nanoparticles to Overcome Multidrug Resistance in Cancer.’ Cancer Res., 67, 4843-4850, (2007).


Product Specific References


This product has been used in:

1. D'Angelo, G., et a, 'Glycosphingolipid synthesis requires FAPP2 transfer of glucosylceramide.' Nature 2007, 449, , 62-67