Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

Metabotropic Glutamate Receptor 2/3

  • Product Code: X1688P
  • Size: 10 Miniblots
  • Availability: In Stock In Stock
  • Price (USD): $335

Cat #

X1688P		 Quantity:      

Data Sheet

Product Name

Metabotropic Glutamate Receptor 2/3

Host/Source

Rabbit

Isotype

IgG

Product Type

Polyclonal Antibody

Reactivity

Rat

Applications

Western Blot, Immunohistochemistry (Frozen)

Purification

Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Size

10 Miniblots

Price (USD)

$335

Background

The metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse (Bhattacharya et al., 2004; Francesconi et al., 2004; Wilson and Nicoll, 2001). Group I metabotropic glutamate receptors (consisting of mGluR1 and mGluR5) are G-protein-coupled neurotransmitter receptors that are localized in the perisynaptic region of the postsynaptic membrane. When activated, Group I mGluRs lead to stimulation of phospholipase and activation of Protein Kinase C. In contrast activation of Group II metabotropic receptors (mGluR2 and mGluR3) leads to inhibition of adenylate cyclase. The mGluR2 subunit has been shown to be required for long-term potentiation at the mossy fiber input in the hippocampus (Yokoi et al., 1996).

Immunogen

Synthetic peptide derived from the C-terminal of the mGluR2 and mGluR3.

Positive Control

HEK-293 cell lysate. Specificity: Specific for the ~110k monomer and the ~220k dimer of mGluR2 and mGluR3. Immunolabeling is blocked by preadsorption of antibody with the peptide used as antigen to generate the antibody.

Formulation

Provided in HEPES (pH 7.5) solution containing 150 mM NaCl, 100 µg per ml BSA and 50% glycerol

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

110 kDa

Product Image

Image Legend

Western blot analysis using mGluR2/3 receptor antibody on HEK-293 cell lysate.

Database Links:

SwissProtP31421Rat

References

1. Bhattacharya M, Babwah AV, Godin C, Anborgh PH, Dale LB, Poulter MO, Ferguson SSG (2004) Ral and phospholipase D2-dependent pathway for constitutive metabotropic glutamate receptor endocytosis. J Neurosci 24:8752-8761.

2. Blaabjerg M, Fang LW, Zimmer J, Baskys A (2003) Neuroprotection against NMDA excitotoxicity by group I metabotropic glutamate receptors is associated with reduction of NMDA stimulated currents. Exp Neurol 183:573-580.

3. Francesconi W, Cammalleri M, Sanna PP (2004) The metabotropic glutamate receptor 5 is necessary for late-phase long-term potentiation in the hippocampal CA1 region. Brain Res 1022:12-18.

4. Wilson RI, Nicoll RA (2001) Endogenous cannabinoids mediate retrograde signalling at hippocampal synapses. Nature (London) 410:588-592.

5. Yokoi M, Kobayashi K, Manabe T, Takahashi T, Sakaguchi I, Katsuura G, Shigemoto R, Ohishi H, Nomura S, Nakamura K, Nakao K, Katsuki M, Nakanishi S (1996) Impairment of hippocampal mossy fiber LTD in mice lacking mGluR2. Science 273:645-647.